Cloning vector allowing mobilization into a wide range of Gramand
Gram+ bacteria. After
mobilization, the plasmid can be maintained by integration into the host chromosome via
homologous recombination. Excision of the intervening plasmid sequence by a double crossover
event can be facilitated by selection on medium containing 10% sucrose.The sacB gene has
been modified to eliminate the HindIII and EcoRI sites in the coding region.
Designation: pK18mobsacB