The Vivid Colors pLenti6.3-V5-GW-EmGFP Expression Control Vector is a 
ViraPower HiPerform positive control lentiviral vector containing 
Emerald Green Fluorescent Protein (EmGFP). It is designed for use 
with the ViraPower HiPerform Lentiviral Expression Systems as a 
positive control to enable the detection of higher levels of 
EmGFP fluorescence following transfection in 293FT cells, as a 
titer control to produce an EmGFP-expressing lentivirus stock 
and as a transduction control following transduction in both 
dividing and non-dividing mammalian cells. The vector has the 
CMV promoter for driving constitutive expression of EmGFP 
and the SV40 promoter for driving expression of the blasticidin 
stable selection marker. The vector is equipped with two key 
genetic elements, making them Hiperform vectors: the Woodchuck 
Posttranscriptional Regulatory Element (WPRE) and the central 
Polypurine Tract (cPPT) sequence from the HIV-1 integrase gene 
to produce at least 4-fold increase in EmGFP expression compared 
to vectors lacking these elements. This control vector is not 
designed for generating EmGFP fusion proteins and does not express 
the V5 epitope. Advantages Lentivirus based expression of EmGFP in dividing and non-dividing 
mammalian cells
Serve as a quick positive control for transfection and lentiviral 
production 
Serve as a quick titer control in determination of lentivirus titer Key Features Constitutive expression with CMV promoter
 High level expression of EmGFP without V5 epitope
 WPRE and cPPT sequences produce at least 4-fold increase in 
EmGFP expression compared to other 

Lenti vectors without these elements
 Blasticidin selection marker for stable selection 

Kit Includes
 pLenti6.3V5-GWEmGFP Expression Control Vector