The pGL4.29[luc2P/CRE/Hygro] Vector(a–f) contains a cAMP response element (CRE) that drives the
transcription of the luciferase reporter gene luc2P (Photinus pyralis). luc2P is a synthetically-derived luciferase sequence
with humanized codon optimization that is designed for high expression and reduced anomalous transcription. The luc2P
gene contains hPEST, a protein destabilization sequence. The protein encoded by luc2P responds more quickly than the protein
encoded by the luc2 gene upon induction. The vector backbone contains an ampicillin resistance gene to allow selection
in E. coli and a mammalian selectable marker for hygromycin resistance.