PAK1 (phospho Thr402) Antibody
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產(chǎn)品名稱: PAK1 (phospho Thr402) Antibody
產(chǎn)品型號:
產(chǎn)品展商: immunoclone
產(chǎn)品文檔: 無相關(guān)文檔
簡單介紹
PAK1 (phospho Thr402) Antibody迅速增長的產(chǎn)品線包括一抗、二抗、**測定及細(xì)胞檢測試劑盒、蛋白/多肽、激動劑/拮抗劑/活化劑/抑制劑及裂解物等。PAK1 (phospho Thr402) Antibody嚴(yán)格的質(zhì)量控制和驗證實驗包括各種不同的應(yīng)用：WB、細(xì)胞**熒光、**組化、流式細(xì)胞術(shù)、ELISA、CHIP、IP和peptide array。
PAK1 (phospho Thr402) Antibody
的詳細(xì)介紹
PAK1 (phospho Thr402) AntibodyProduct Profile
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Product Name
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PAK1 (phospho Thr402) Antibody
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Antibody Type
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Primary Antibodies
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Product description
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In mammals, there are several identified isoforms of p21-activated protein kinases or PAKs: alpha-PAK (also known as PAK-1) and beta-PAK (also known as PAK-3) are mostly brain-specific, while -PAK (also known as PAK-2) is expressed ubiquitously (Jakobi et al., 2003). Mutations of the gene coding for PAK-3 are associated with X-linked mental retardation and recent work indicates that PAK-3 is a key regulator of synapse formation and plasticity in the hippocampus (Boda et al., 2004). PAK-3 is thought to play a key role in regulation of cell shape and motility as well as cell death (Jakobi et al., 2003; Walter et al., 1998). Autophosphorylation of Thr402 in the protein has been found to be essential for activation of PAK (Jakobi et al., 2000).1) Boda B, Alberi S, Nikonenko I, Node-Langlois R, Jourdain P, Moosmayer M, Parisi-Jourdain L, Muller D (2004) The mental retardation protein PAK-3 contributes to synapse formation and plasticity in hippocampus. J Neurosci 24:10816-10825.
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Immunogen
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Phosphopeptide corresponding to amino acid residues surrounding the phospho-Thr402 of rat p21 activated kinase 2 (PAK-2). The peptide sequence used is identical in PAK-1, 2 and 3. Note: Thr402 in PAK-2 corresponds to Thr423 in human PAK-1.
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PAK1 (phospho Thr402) AntibodyKey Feature
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Clonality
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Polyclonal
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Host Species
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Rabbit
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Tested Applications
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WB
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The antibody has been directly tested for reactivity in Western blots with rat tissue. It is anticipated that the antibody will react with bovine, canine, human and mouse based on the fact that these species have 100% homology with the amino acid sequence used as antigen.:
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Species Reactivity
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BovineDogHumanMouseRat
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Concentration
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1mg/ml
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Purification
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Affinity purified
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PAK1 (phospho Thr402) AntibodyTarget Information
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Gene Symbol
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Pak1
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Alternative Names
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Alpha-PAK
PAK-1
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Molecular Weight(MW)
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68/70
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PAK1 (phospho Thr402) AntibodyDatabase Links
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Entrez Gene
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29431
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Protein Accession
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P35465
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PAK1 (phospho Thr402) AntibodyApplication
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Application
Western blot of rat hippocampal lysate showing specific immunolabeling of the ~68k to ~70k PAK protein (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: lamda-Ptase). The blot is identical to the control except that it was incubated in lamda-Ptase (1200 units for 30 min) before being exposed to the phospho-Thr402 PAK-1,2,3 antibody. The immunolabel
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Application Notes
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The antibody has been directly tested for reactivity in Western blots with rat tissue. It is anticipated that the antibody will react with bovine, canine, human and mouse based on the fact that these species have 100% homology with the amino acid sequence used as antigen.:
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Additional Information
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Form
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Liquid
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Storage Instructions
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PAK1 antibody can be stored at -20?C and is stable at -20?C for at least 1 year.
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Storage Buffer
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100 uL in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per mL BSA and 50% glycerol.
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