Tyrosine Hydroxylase (phospho Ser40) Antibody
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產(chǎn)品名稱: Tyrosine Hydroxylase (phospho Ser40) Antibody
產(chǎn)品型號:
產(chǎn)品展商: immunoclone
產(chǎn)品文檔: 無相關(guān)文檔
簡單介紹
Tyrosine Hydroxylase (phospho Ser40) Antibody迅速增長的產(chǎn)品線包括一抗、二抗、**測定及細(xì)胞檢測試劑盒、蛋白/多肽、激動劑/拮抗劑/活化劑/抑制劑及裂解物等。Tyrosine Hydroxylase (phospho Ser40) Antibody嚴(yán)格的質(zhì)量控制和驗(yàn)證實(shí)驗(yàn)包括各種不同的應(yīng)用：WB、細(xì)胞**熒光、**組化、流式細(xì)胞術(shù)、ELISA、CHIP、IP和peptide array。
Tyrosine Hydroxylase (phospho Ser40) Antibody
的詳細(xì)介紹
Tyrosine Hydroxylase (phospho Ser40) AntibodyProduct Profile
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Product Name
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Tyrosine Hydroxylase (phospho Ser40) Antibody
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Antibody Type
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Primary Antibodies
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Product description
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Tyrosine Hydroxylase (TH) is the rate-limiting enzyme in the synthesis of the catecholamines Dopamine and Norepinephrine. TH antibodies can therefore be used as markers for dopaminergic and noradrenergic neurons. We raised this polyclonal antibody against a peptide representing the sequence around Ser40 in rat TH. This antibody is suitable for most immunochemical applications in a variety of mammalian and some non-mammalian species.1) Witkovsky, P., Gabriel, R., Haycock, J. W., and Meller, E., "Influence of light and neural circuitry on tyrosine hydroxylase phosphorylation in the rat retina," J. Chem. Neuroanat., 19 (2000) 105 - 116.
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Immunogen
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TH (Ser40) polyclonal antibody was raised against a synthetic phosphopeptide corresponding to amino acids residues surrounding the phospho-Ser40 of rat tyrosine hydroxylase (TH).
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Tyrosine Hydroxylase (phospho Ser40) AntibodyKey Feature
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Clonality
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Polyclonal
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Host Species
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Rabbit
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Tested Applications
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IFIHCWB
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Applications include Immunofluorescence (IF), Immunohistochemistry (IHC) and Western Blots (WB). Human, porcine, quail, mouse, and non-human primate have 100% amino acid sequence identity with the antigen used to raise the antibody. When internally tested under ideal conditions the working dilutions were 1:1000 for IF, IHC and WB.:
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Species Reactivity
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HumanMonkeyMousePorcine
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Concentration
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1mg/ml
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Purification
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Affinity purified
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Tyrosine Hydroxylase (phospho Ser40) AntibodyTarget Information
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Gene Symbol
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Th
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Alternative Names
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The
Tyrosine 3-hydroxylase
TH
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Molecular Weight(MW)
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60
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Tissue Specificity
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Tyrosine Hydroxylase antibody is specific for the 60k rat TH phosphorylated at Ser40 in lysates of PC-12 Cells stimulated by Okadaic Acid (1 μM for 60 minutes). Some higher molecular weight bands may be detected depending upon the brain region being studied, protein loads, and the detection methods used. See cited reference.
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Tyrosine Hydroxylase (phospho Ser40) AntibodyDatabase Links
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Entrez Gene
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25085
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Protein Accession
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P04177
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Tyrosine Hydroxylase (phospho Ser40) AntibodyApplication
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Application
Western Blots of phospho- and dephospho-proteins to demonstrate the selectivity of the Phospho-Specific Antibody. The Pan-Specific Antibody recognized both the dephospho-Tyrosine Hydroxylase (dephospho-TH) and the phospho-Tyrosine Hydroxylase (phospho-TH). Most importantly, the Phospho-Specific Antibody recognized only phospho-TH whereas the Dephospho-Specific Antibody reacted selectively with dep
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Application Notes
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Applications include Immunofluorescence (IF), Immunohistochemistry (IHC) and Western Blots (WB). Human, porcine, quail, mouse, and non-human primate have 100% amino acid sequence identity with the antigen used to raise the antibody. When internally tested under ideal conditions the working dilutions were 1:1000 for IF, IHC and WB.:
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Additional Information
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Form
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Liquid
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Storage Instructions
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For long term storage –80?C is recommended, but shorter term storage at –20?C is also acceptable as aliquots may be taken without freeze/thawing due to the presence of 50% glycerol. Stable for one year.
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Storage Buffer
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100 uL in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per mL BSA and 50% glycerol.
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