4E BP1 (phospho Thr45) Antibody
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產品名稱: 4E BP1 (phospho Thr45) Antibody
產品型號:
產品展商: immunoclone
產品文檔: 無相關文檔
簡單介紹
4E BP1 (phospho Thr45) Antibody迅速增長的產品線包括一抗、二抗、**測定及細胞檢測試劑盒、蛋白/多肽、激動劑/拮抗劑/活化劑/抑制劑及裂解物等。4E BP1 (phospho Thr45) Antibody嚴格的質量控制和驗證實驗包括各種不同的應用：WB、細胞**熒光、**組化、流式細胞術、ELISA、CHIP、IP和peptide array。
4E BP1 (phospho Thr45) Antibody
的詳細介紹
4E BP1 (phospho Thr45) AntibodyProduct Profile
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Product Name
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4E BP1 (phospho Thr45) Antibody
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Antibody Type
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Primary Antibodies
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Product description
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4E-BP1 encodes one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation.1) Gingras AC, et al. (1998) Genes Dev 12 (4): 502-513. Brugarolas J, et al. (2004) Genes Dev 18 (23): 2893-2904. Kumar V, et al. (2000) EMBO J 19 (5): 1087-1097. Moody CA, et al. (2005) J Virol 79 (9): 5499-5506. Burnett PE, et al. (1998) Proc Natl Acad Sci U S A 95 (4): 1432-1437.
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Immunogen
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4E-BP1 (Phospho-Thr45) antibody was raised against a peptide sequence around phosphorylation site of threonine 45 (S-T-T (p) -P-G) derived from Human 4E-BP1.
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4E BP1 (phospho Thr45) AntibodyKey Feature
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Clonality
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Polyclonal
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Host Species
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Rabbit
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Tested Applications
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IHCWB
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Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100:
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Species Reactivity
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Human
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Concentration
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1mg/ml
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Purification
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Affinity purified
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4E BP1 (phospho Thr45) AntibodyTarget Information
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Gene Symbol
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EIF4EBP1
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Alternative Names
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BP-1
4EBP1
4E-BP1
PHAS-I
STK14A
EIF4EBP1
PHAS-1
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Molecular Weight(MW)
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18 kDa
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Tissue Specificity
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This antibody detects endogenous level of 4E-BP1 only when phosphorylated at Threonine 45.
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4E BP1 (phospho Thr45) AntibodyDatabase Links
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Entrez Gene
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1978
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Protein Accession
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NP_004086.1
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4E BP1 (phospho Thr45) AntibodyApplication
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Application
Western blot analysis of lysed extracts from HeLa cells using 4E-BP1 (Phospho-Thr45).
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Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using 4E-BP1 (Phospho-Thr45).
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Application Notes
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Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100:
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Additional Information
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Form
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Liquid
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Storage Instructions
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Store antibody at -20?C for up to one year.
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Storage Buffer
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Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
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