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產品名稱: StrepII-tag Antibody
產品型號:
產品展商: immunoclone
產品文檔: 無相關文檔
簡單介紹
StrepII-tag Antibody迅速增長的產品線包括一抗、二抗、**測定及細胞檢測試劑盒、蛋白/多肽、激動劑/拮抗劑/活化劑/抑制劑及裂解物等。StrepII-tag Antibody嚴格的質量控制和驗證實驗包括各種不同的應用：WB、細胞**熒光、**組化、流式細胞術、ELISA、CHIP、IP和peptide array。
StrepII-tag Antibody
的詳細介紹
StrepII-tag AntibodyProduct Profile
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Product Name
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StrepII-tag Antibody
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Antibody Type
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Tags Antibodies
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Product description
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StrepII-tag Antibody: Streptavidin is a tetrameric protein purified from Streptomyces avidinii. It has wide use in numerous molecular biological protocols dues to its strong affinity for biotin. The original Strep-tag is a nine amino acid peptide with high specificity and affinity towards streptavidin. The addition of this tag to the C-terminus of recombinant proteins allowed the simple purification of protein by use of affinity columns. It was re-engineered to allow it to also placed at the N-terminus of recombinant proteins.1) Schmidt TGM and Skerra A. The random peptide-assisted engineering of a C-terminal affinity peptide, useful for the detection and purification of a functional Ig Fv fragment. Protein Eng.1993; 6:109-22.
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Immunogen
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StrepII-tag antibody was raised against the amino acid sequence "asWSHPQFEKga".
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StrepII-tag AntibodyKey Feature
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Clonality
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Polyclonal
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Isotype
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IgG
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Host Species
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Rabbit
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Tested Applications
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ELISAWB
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StrepII-tag antibody can be used for the detection of StrepII-tagged proteins by Western blot at 1 μg/mL.:
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Species Reactivity
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Tag
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Concentration
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1mg/ml
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Purification
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Affinity purified
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StrepII-tag AntibodyTarget Information
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Alternative Names
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StrepII-tag Antibody:
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Database Links
StrepII-tag AntibodyApplication
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Application
Western blot analysis of (A) 5 and (B) 25ng of StrepII-tagged recombinant protein with StrepII antibody at 1 μg/mL.
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Application Notes
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StrepII-tag antibody can be used for the detection of StrepII-tagged proteins by Western blot at 1 μg/mL.:
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StrepII-tag AntibodyAdditional Information
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Form
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Liquid
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Storage Instructions
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StrepII-tag antibody can be stored at 4?C for three months and -20?C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Storage Buffer
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StrepII-tag Antibody is supplied in PBS containing 0.02% sodium azide.
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