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產品名稱: 6X His tag antibody
產品型號:
產品展商: immunoclone
產品文檔: 無相關文檔
簡單介紹
6X His tag antibody迅速增長的產品線包括一抗、二抗、**測定及細胞檢測試劑盒、蛋白/多肽、激動劑/拮抗劑/活化劑/抑制劑及裂解物等。6X His tag antibody嚴格的質量控制和驗證實驗包括各種不同的應用：WB、細胞**熒光、**組化、流式細胞術、ELISA、CHIP、IP和peptide array。
6X His tag antibody
的詳細介紹
6X His tag antibodyProduct Profile
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Product Name
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6X His tag antibody
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Antibody Type
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Tags Antibodies
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Product description
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Rabbit Polyclonal antibody to 6X His tag
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6X His tag antibodyKey Feature
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Clonality
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Polyclonal
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Isotype
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IgG
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Host Species
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Rabbit
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Tested Applications
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ELISAIHC-WIPWB
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*Optimal dilutions/concentrations should be determined by the researcher.:
WB:1:500~1:2000
IP:1:100~1:500
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Concentration
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1 mg/ml (Please refer to the vial label for the specific concentration)
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Purification
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Affinity purified
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6X His tag antibodyTarget Information
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Gene Synonyms
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6X His tag antibody
His antibody
6x His antibody
6x his tag antibody
His Tag antibody
6xHis Tag antibody
6 x His antibody
More
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6X His tag antibodyDatabase Links
6X His tag antibodyApplication
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Application
6X His tag antibody detects His-tagged recombinant proteins by indirect ELISA analysis. Various dilutions of His-tagged recombinant proteins coated onto the 96-well plate were detected by using 2.5 μg/ml 6x His antibody . Bound Abs were further detected by using HRP-conjugated goat anti-rabbit IgG with HRP substrates.
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Application
Sample A: 10 μg of non-transformed E. coli lysate B: 10 μg of non-transformed E. coli lysate, induced C: 10ug E.coli Lysate harbouring His-tagged recombinant protein (N-terminal linked), non-induced D: 10ug E.coli Lysate harbouring His-tagged recombinant protein (N-terminal linked), induced 12% SDS PAGE diluated at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
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Application
His-tagged protein was immunoprecipitated from transfected cells using 8 ug of 6X His tag antibody . The precipitated His-tagged protein was detected by diluted at 1:500, and used EasyBlot anti-rabbit IgG as secondary reagent.
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Application
Western blot analysis of extracts from untransfected control cells and transfected cells overexpressing His-tagged protein (~19 kDa), using 6X His tag antibody. A: 10ug E.coli Lysate harbouring His-tagged recombinant protein (N-terminal linked), non-induced (10 μg). B: 10ug E.coli Lysate harbouring His-tagged recombinant protein (N-terminal linked), induced (10 μg). The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
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Positive Control
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His-tagged recombinant protein
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Application Notes
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*Optimal dilutions/concentrations should be determined by the researcher.:
WB:1:500~1:2000
IP:1:100~1:500
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Additional Information
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Form
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Liquid
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Storage Instructions
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Keep as concentrated solution. Aliquot and store at -20oC or below. Avoid multiple freeze-thaw cycles.
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Storage Buffer
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1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
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