KHOS-321H細胞

運輸方式： 凍存運輸

生長狀態： 貼壁生長

年限： 13 years

數量： 大量

ATCC Number： CRL-1546?

相關**： 骨肉瘤

器官來源： 骨

是否是腫瘤細胞： 1

物種來源： 人

Designations: KHOS-312H

Depositors: JS Rhim

KHOS-321H細胞Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens

Morphology:

Source: Organ: bone

Disease: osteosarcoma

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Tumorigenic: No

DNA Profile (STR): Amelogenin: X

CSF1PO: 12

D13S317: 12

KHOS-321H細胞D16S539: 10,13

D5S818: 13

D7S820: 11,12

THO1: 6

TPOX: 8,11

vWA: 18

Age: 13 years

Gender: female

Ethnicity: Caucasian

Comments: The growth properties of KHOS-312H are similar to HOS (ATCC CRL-1543).

This line is a revertant of ATCC CRL-1544 that no longer has a rescuable Kirsten murine sarcoma virus genome.

Note: An inconsistency in our records was discovered late in 2010 concerning the human osteosarcoma cell line, KHOS-312H. Due to an ATCC catalog transfer error, this cell line has been referred to as both KHOS-312H and KHOS-321H by ATCC and in the literature. The name originally supplied to ATCC by the depositor was KHOS-312H.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. KHOS-321H細胞To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:4 is recommended

Medium Renewal: 2 to 3 times per week

Remove medium, rinse with fresh 0.25% trypsin-0.53 mM EDTAsolution, remove trypsin and let the culture sit at 37C for 10 to 12 minutes or until cells detach. Add fresh medium, aspirate and dispense into new flasks. Subculture every 7 days.

References: 22503: Yang YH, et al. Reversion of Kirsten sarcoma virus transformed human cells: elimination of the sarcoma virus nucleotide sequences. J. Gen. Virol. 43: 447-451, 1979. PubMed: 225428

26080: Cho HY, et al. Revertants of human cells transformed by murine sarcoma virus. Science 194: 951-953, 1976. PubMed: 62397

26082: Cho HY, Rhim JS. Cycloheximide-dependent reversion of human cells transformed by MSV and chemical carcinogen. Science 205: 691-693, 1979.KHOS-321H細胞 PubMed: 223242