Hs 683細(xì)胞

器官來源： 大腦

生長(zhǎng)狀態(tài)： 貼壁生長(zhǎng)

年限： 76 years

ATCC Number： HTB-138?

相關(guān)**： 神經(jīng)膠質(zhì)瘤

細(xì)胞形態(tài)： 成纖維樣

運(yùn)輸方式： 凍存運(yùn)輸

數(shù)量： 大量

是否是腫瘤細(xì)胞： 1

物種來源： 人

Designations: Hs 683

Depositors: RB Owens

Hs 683細(xì)胞Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens

Morphology: fibroblast

Source: Organ: brain

Disease: glioma

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Tumorigenic: No

DNA Profile (STR): Amelogenin: X,Y

CSF1PO: 9,13

D13S317: 8,12

D16S539: 9,10

Hs 683細(xì)胞D5S818: 11,12

D7S820: 11

THO1: 6,8

TPOX: 8,11

vWA: 18,20

Cytogenetic Analysis: modal number = 82; range = 74 to 87.

This is a hypertriploid human cell line with the modal chromosome number of 82 occurring in 16% of cells, with the distribution skewed toward the lower number range. Higher ploidies occurred in 3.0% of cells. Most cells had 4-6 marker chromosomes, but only del(3) (q12.2q21) occurred frequently in most cells, and der(9)t(9;?) (q34.2;?), tdic(1;4) (q21;q35), and a minute acrocentric occurred in more than two cells. N7 occurred in 4-6 copies per cell. Two X and one or two Y chromosomes were present in most cells.

Isoenzymes: AK-1, 1

ES-D, 1

G6PD, B

GLO-I, 2

Me-2, 2

PGM1, 1

PGM3, 1-2

Age: 76 years

Gender: male

Ethnicity: Caucasian

Comments: Hs 683 was isolated from explant cultures of a glioma taken from the left temporal lobe of a 76 year old male Caucasian. Microvilli but no desmosomes were observed.

Propagation: Hs 683細(xì)胞ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Temperature: 37.0°C

Subculturing: Protocol:

Remove and discard culture medium.

Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.

Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37?C to facilitate dispersal.

Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

Add appropriate aliquots of the cell suspension to new culture vessels.

Incubate cultures at 37?C.

Subcultivation Ratio: A subcultivation ratio of 1:4 is recommended

Medium Renewal: Twice per week

Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO

Storage temperature: liquid nitrogen vapor phase

References: 22534: Owens RB, et al. Epithelial cell cultures from normal and cancerous human tissues. J. Natl. Cancer Inst. 56: 843-849, 1976. PubMed: 176412

23094: Olopade OI, et al. Molecular analysis of deletions of the short arm of chromosome 9 in human gliomas. Cancer Res. 52: 2523-2529, 1992. PubMed: 1568221

23221: Gershwin ME, et al. Immunobiology of heterotransplanted human tumors in nude mice. J. Natl. Cancer Inst. 58: 1455-1463, 1977. PubMed: 857033

32550: Debinski W, et al. Hs 683細(xì)胞Receptor for interleukin (IL) 13 does not interact with IL4 but receptor for IL4 interacts with IL13 on human glioma cells. J. Biol. Chem. 271: 22428-22433, 1996. PubMed: 8798406