NCI-H650細(xì)胞
器官來(lái)源： 肺
ATCC Number： CRL-5835?
相關(guān)**： 其他**
細(xì)胞形態(tài)： 其他
生長(zhǎng)狀態(tài)： 懸浮生長(zhǎng)，多細(xì)胞聚集
數(shù)量： 大量
運(yùn)輸方式： 凍存運(yùn)輸
是否是腫瘤細(xì)胞： 1
物種來(lái)源： 人
Designations: NCI-H650 [H650]
Depositors: AF Gazdar, JD Minna
Biosafety Level: 1
NCI-H650細(xì)胞Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension, multicell aggregates
Organism: Homo sapiens
Morphology: spherical
Source: Organ: lung
Disease: bronchioalveolar carcinoma; non-small cell lung cancer
Derived from metastatic site: lymph node
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Restrictions: The line is available with the following restrictions: 1. This cell line was deposited at the ATCC by Dr. A. Gazdar and Dr. J. Minna and is provided for research purposes only. Neither the cell line nor products derived from it may be sold or used for commercial purposes. Nor can the cells be distributed to third parties for purposes of sale, or producing for sale, cells or their products. The cells are provided as service to the research community. They are provided without warranty of merchantability or fitness for a particular purpose or any other warranty, expressed or implied. 2. NCI-H650細(xì)胞Any proposed commercial use of the these cells, or their products must first be negotiated with the University of Texas Southwestern Medical Center at Dallas, 5323 Harry Hines Blvd., Dallas, Texas 75235. Telephone (214) 699-8056, FAX (214) 688-7233.
Isolation: Isolation date: October, 1983
DNA Profile (STR): Amelogenin: X
CSF1PO: 10
D13S317: 8,12
D16S539: 8,11
D5S818: 11
D7S820: 10,11
THO1: 6,9.3
TPOX: 8,11
vWA: 17
Cytogenetic Analysis: modal number = 90; range = 54 to 178; del(p14-p23), inv(3p)
Gender: male
Comments: The tissue donor was a non-smoker.
Propagation: NCI-H650細(xì)胞ATCC complete growth medium: ACL-4 medium (serum-free)
The base medium for this cell line is ATCC formulated DMEM: F12 Medium Catalog No. 30-2006. To make the complete growth medium, add the following components to the base medium:
0.02 mg/ml insulin
0.01 mg/ml transferrin
25 nM sodium selenite (final conc.)
50 nM Hydrocortisone (final conc.)
1 ng/ml Epidermal Growth Factor (do not filter)
0.01 mM ethanolamine (final conc.)
0.01 mM phosphorylethanolamine (final conc.)
100 pM triiodothyronine (final conc.)
0.5% (w/v) bovine serum albumin (final conc.)
0.5 mM sodium pyruvate (final conc.)
extra 2mM L-glutamine (for final conc. of 4.5mM)
Temperature: 37.0°C
Subculturing: Protocol: Cells can be maintained by the addition of fresh medium.
Preservation: Freeze medium: Complete growth medium, 92.5%; DMSO, 7.5%
Storage temperature: liquid nitrogen vapor temperature
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2006
References: 23570: . NCI-H650細(xì)胞NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996.
