NCI-H1694細胞
細胞形態(tài)： 其他
ATCC Number： CRL-5888?
相關**： 小細胞肺癌
是否是腫瘤細胞： 1
物種來源： 人
數(shù)量： 大量
年限： stage E
運輸方式： 凍存運輸
器官來源： 肺
生長狀態(tài)： 懸浮生長
Designations: NCI-H1694 [H1694]
Depositors: AF Gazdar, JD Minna
NCI-H1694細胞Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Homo sapiens
Morphology: floating clusters
Source: Organ: lung
Tumor Stage: stage E
Disease: carcinoma; classic small cell lung cancer
Derived from metastatic site: ascites
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Restrictions: NCI-H1694細胞The line is available with the following restrictions: 1. This cell line was deposited at the ATCC by Dr. A. Gazdar and Dr. J. Minna and is provided for research purposes only. Neither the cell line nor products derived from it may be sold or used for commercial purposes. Nor can the cells be distributed to third parties for purposes of sale, or producing for sale, cells or their products. The cells are provided as service to the research community. They are provided without warranty of merchantability or fitness for a particular purpose or any other warranty, expressed or implied. 2. Any proposed commercial use of the these cells, or their products must first be negotiated with the University of Texas Southwestern Medical Center at Dallas, 5323 Harry Hines Blvd., Dallas, Texas 75235. Telephone (214) 699-8056, FAX (214) 688-7233.
DNA Profile (STR): Amelogenin: X
CSF1PO: 12
D13S317: 11
D16S539: 9,11
D5S818: 12
D7S820: 11
THO1: 6,9
TPOX: 11
vWA: 15,16
Cytogenetic Analysis: del(p21-pter)
Age: 61 years
Gender: male
Ethnicity: Caucasian
Comments: NCI-H1694細胞The line was established in July 1987.
The tissue donor was a non-smoker.
Propagation: ATCC complete growth medium: HITES medium supplemented with 5% fetal bovine serum
The base medium for this cell line is ATCC-formulated DMEM:F12 Medium Catalog No.30-2006. To make the complete growth medium,add the following components to the base medium
0.005 mg/ml Insulin
0.01 mg/ml Transferrin
30nM Sodium selenite (final conc.)
10 nM Hydrocortisone (final conc.)
10 nM beta-estradiol (final conc.)
extra 2mM L-glutamine (for final conc. of 4.5 mM)
5% fetal bovine serum (final conc.)
Temperature: 37.0°C
Subculturing: Cultures can be maintained by the addition of fresh mediumor replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension in fresh medium. Cultures grow as floating aggregates of large clusters of cells. Some cells may attach. NCI-H1694細胞Add medium as cell density increases.
Preservation: Culture medium, 95%; DMSO, 5%
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2006
References: 23570: . NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996.
