NCI-H661細(xì)胞
ATCC Number： HTB-183?
相關(guān)**： 其他**
運輸方式： 凍存運輸
年限： 43 years
細(xì)胞形態(tài)： 上皮樣
是否是腫瘤細(xì)胞： 1
物種來源： 人
數(shù)量： 大量
生長狀態(tài)： 貼壁生長
器官來源： 肺
Designations: NCI-H661 [H661]
Depositors: AF Gazdar, JD Minna
NCI-H661細(xì)胞Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens
Morphology: epithelial
Source: Organ: lung
Disease: carcinoma; large cell lung cancer
Derived from metastatic site: lymph node
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Isolation: Bethesda Maryland,
Isolation date: 1982
DNA Profile (STR): Amelogenin: X,Y
CSF1PO: 10
D13S317: 11
D16S539: 12
NCI-H661細(xì)胞D5S818: 11
D7S820: 8,10
THO1: 8
TPOX: 8
vWA: 17
Cytogenetic Analysis: hyperhexaploid; modal number = 142; range = 130 to 153. Each cell had over 70 marker chromosomes. Among the markers were t(1p10q), del(1)(q11/q12), 6q+, 2q+,der(20)t(12;20)(q11;q13). Some of these had 2 to copies per cell. Most cells also had 5 to 10 microchromosomes (metacentric like). Structurally normal N2,N4 and N9 were absent; two copies of the X and multiple copies of the Y were detected.
Isoenzymes: AK-1, 1
ES-D, 1
G6PD, B
GLO-I, 1-2
Me-2, 0
PGM1, 1
PGM3, 1
Age: 43 years
Gender: male
Ethnicity: Caucasian
Comments: The line lacks ultrastructural and biochemical evidence of squamous differentiation or mucin production.
The cells express easily detectable p53 mRNA at levels comparable to normal lung tissue, and exhibit no gross structural DNA abnormalities.
The cells stain positively for keratin and vimentin but are negative for neurofilament triplet protein.
Propagation: NCI-H661細(xì)胞ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Subculturing: Protocol: Remove medium, add fresh 0.25% trypsin, 0.53 mM EDTA, rinse quickly and remove trypsin leaving 1 to 2 ml. Let the culture sit at room temperature (or at 37C) until the cells detach. Add fresh medium, disperse cells and transfer to a new flask.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:5 is recommended
Medium Renewal: 2 to 3 times per week
Preservation: Freeze medium: Culture medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
References: 1605: Banks-Schlegel SP, et al. Intermediate filament and cross-linked envelope expression in human lung tumor cell lines. Cancer Res. 45: 1187-1197, 1985. PubMed: 2578876
1806: Takahashi T, et al. p53: A frequent target for genetic abnormalities in lung cancer. Science 246: 491-494, 1989. PubMed: 2554494
22437: Levitt ML, et al. NCI-H661細(xì)胞Cross-linked envelope-related markers for squamous differentiation in human lung cancer cell lines. Cancer Res. 50: 120-128, 1990. PubMed: 1967140
