TOV-112D細(xì)胞
生長(zhǎng)狀態(tài)： 貼壁生長(zhǎng)
細(xì)胞形態(tài)： 上皮樣
運(yùn)輸方式： 凍存運(yùn)輸
數(shù)量： 大量
年限： grade 3, stage IIIC
ATCC Number： CRL-11731?
相關(guān)**： 其他**
是否是腫瘤細(xì)胞： 1
物種來(lái)源： 人
器官來(lái)源： 卵巢
Designations: TOV-112D
Depositors: University of Montreal
TOV-112D細(xì)胞Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens deposited as human
Morphology: epithelial
Source: Organ: ovary
Tumor Stage: grade 3, stage IIIC
Disease: primary malignant adenocarcinoma; endometrioid carcinoma
Cellular Products: keratin [49408]
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Tumorigenic: Yes
Oncogene: her2/neu +, p53 (mutated, Arg --> His mutation at exon 6, codon 175)
DNA Profile (STR): TOV-112D細(xì)胞Amelogenin: X
CSF1PO: 12
D13S317: 8
D16S539: 9,12
D5S818: 10
D7S820: 9,10
THO1: 6
TPOX: 8,11
vWA: 18
Cytogenetic Analysis: 52, XX, add(X)(p22), +add(1)(p22), +add(1)(p22), +2, +9, +12, add(15)(p11), +17 [49408]
Age: 42 years *****
Gender: female
Comments: This cell line was initiated in October of 1992 from a patient with early onset ovarian cancer. The patient was of French-Canadian descent with an unknown family history of ovarian cancer. [49408]
Unlike TOV-21G (ATCC CRL-11730) and OV-90 (ATCC CRL-11732), the TOV-112D cell line does not exhibit a deletion at chromosome 3p24. [42090]
TOV-112D細(xì)胞Propagation: ATCC complete growth medium: The base medium for this cell line is a 1:1 mixture of MCDB 105 medium containing a final concentration of 1.5 g/L sodium bicarbonate and Medium 199 containing a final concentration of 2.2 g/L sodium bicarbonate. To make the complete growth medium, add the following components to the base medium:
fetal bovine serum to a final concentration of 15%
Temperature: 37.0°C
Subculturing: Protocol: Remove medium, add fresh 0.05% trypsin - 0.53 mM EDTA, rinse and remove trypsin. Allow the culture sit at room temperature (or 37C) until the cells detach. Add fresh medium, aspirate and dispense into new flasks.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended
Medium Renewal: Every 3 to 4 days
Preservation: Freeze medium: Complete growth medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor temperature
Related Products: recommended serum:ATCC 30-2020
purified DNA:ATCC CRL-11731
References: 42090: Mes-Masson AM, Provencher D. Primary cultures of normal and tumoral human ovarian epithelium. US Patent 5,710,038 dated Jan 20 1998
49408: Provencher DM, et al. TOV-112D細(xì)胞Characterization of four novel epithelial cancer cell lines. In Vitro Cell. Dev. Biol. Anim. 36: 357-361, 2000. PubMed: 10949993
