HH細(xì)胞
細(xì)胞類型： 其他細(xì)胞類型
是否是腫瘤細(xì)胞： 1
物種來源： 人
生長狀態(tài)： 懸浮生長
數(shù)量： 大量
年限： 61 years *****
ATCC Number： CRL-2105?
運(yùn)輸方式： 凍存運(yùn)輸
相關(guān)**： 其他**
細(xì)胞形態(tài)： **樣
器官來源： 外周血
HH細(xì)胞Designations: HH
Depositors: G Starkebaum
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Homo sapiens
Morphology: lymphoblast
Source: Organ: peripheral blood
Disease: cutaneous T cell lymphoma
Cell Type: lymphocyte T lymphocyte;
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Receptors: HH細(xì)胞interleukin 2 (IL-2) beta chain, expressed
Reverse Transcript: N
Antigen Expression: CD2; Homo sapiens, expressed
CD3; Homo sapiens, expressed
CD4; Homo sapiens, expressed
CD8; Homo sapiens; negative
CD25; Homo sapiens; negative
CD30; Homo sapiens, expressed
HLA-DR; Homo sapiens, expressed
DNA Profile (STR): Amelogenin: X
CSF1PO: 11,12
D13S317: 11,12
D16S539: 12
D5S818: 11,13
D7S820: 10
THO1: 9
TPOX: 8,11
vWA: 15,16
Age: 61 years *****
Gender: male
Ethnicity: HH細(xì)胞Caucasian
HeLa Markers: N
Comments: The line was isolated from a patient who was negative for anti human T lymphotropic virus type I (HTLV-I) antibody, and no HTLV-I sequences were detected in the cultured cells by Southern analysis. Although HH cells lack the alpha chain of the IL-2 receptor (IL-2R alpha), they do possess a functional p70 beta chain (IL-2R beta) of the IL-2 receptor complex.
A culture submitted to the ATCC in June of 1992 was found to be contaminated with mycoplasma, and was cured by treatment with Ciprofloxacin.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Subculturing: Protocol: Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10(5) cells/ml and maintain between 1 X 10(5) and 1 X 10(6) cells/ml.
Medium Renewal: Every 2 to 3 days
Preservation: Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
References: 22863: Starkebaum G, et al. HH細(xì)胞Establishment of an IL-2 independent, human T-cell line possessing only the p70 IL-2 receptor. Int. J. Cancer 49: 246-253, 1991. PubMed: 1879969
