SW 954細胞

ATCC Number： HTB-117?

相關**： 鱗狀細胞癌

運輸方式： 凍存運輸

器官來源： 其他

年限： grade II

生長狀態： 貼壁生長

數量： 大量

細胞形態： 上皮樣

是否是腫瘤細胞： 1

物種來源： 人

Designations: SW 954 [SW-954, SW954]

SW 954細胞Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens

Morphology: epithelial

Source: Organ: vulva

Tumor Stage: grade II

Disease: squamous cell carcinoma

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Antigen Expression: blood type A; Rh-

DNA Profile (STR): Amelogenin: X

CSF1PO: 10,11

D13S317: 9,11

D16S539: 11

D5S818: 11,12

D7S820: 11,13

SW 954細胞THO1: 8,9.3

TPOX: 8

vWA: 14,15

Cytogenetic Analysis: pseudodiploid; modal number = 46. The rate of higher ploidies was 16%. The t(3q;11p), der(11)t(11;?)(q13;?), and M3 were common to all cells., The der(1)t(1;2)(p13;q11) and two others were seen in some cells, and about 10 others were seen only once. All these marker chromosomes were present in one copy per cell., Normal N3, N9, N11 and N18 were single copied, and others were paired. The X was also paired.

Isoenzymes: AK-1, 1

ES-D, 1-2

G6PD, B

GLO-I, 1-2

PGM1, 1

PGM3, 1-2

Age: 86 years

Gender: female

Ethnicity: Caucasian

Comments: The SW 954 cell line was initiated by A. Leibovitz in April 1975 at the Scott and White Clinic, Temple, Texas from biopsy tissue of a squamous cell carcinoma of the vulva of an 86 year old female Caucasian.

The histopathology of the surgical specimen was determined at the Scott and White Clinic to be a grade II carcinoma.

A frozen ampule of the line at passage 5 was transferred to the ATCC in January, 1982.

Propagation: SW 954細胞ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Leibovitz's L-15 Medium, Catalog No. 30-2008. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Atmosphere: air, 100%

Subculturing: Protocol: Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. 1. Remove and discard culture medium.2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum, which contains trypsin inhibitor.3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 10 minutes).Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37?C to facilitate dispersal.4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting. 5. Add appropriate aliquots of the cell suspension to new culture vessels. 6. Incubate cultures at 37?C.

Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended

Medium Renewal: 2 to 3 times per week

Preservation: Culture medium, 95%; DMSO, 5%

References: 27819: Goodrum FD, Ornelles DA. The early region 1B 55-kilodalton oncoprotein of adenovirus relieves growth restrictions imposed on viral replication by the cell cycle. J. Virol. 71: 548-561, 1997. SW 954細胞PubMed: 8985383