T24細胞

生長狀態： 貼壁生長

年限： 81 years

細胞形態： 上皮樣

數量： 大量

器官來源： 膀胱

是否是腫瘤細胞： 1

物種來源： 人

運輸方式： 凍存運輸

ATCC Number： HTB-4?

相關**： 移行細胞癌

Designations: T24

Depositors: C O'Toole

T24細胞Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens

Morphology: epithelial

Source: Organ: urinary bladder

Disease: transitional cell carcinoma

Cellular Products: tumor specific antigen

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Applications: transfection host

Tumorigenic: Yes

Antigen Expression: HLA A1, A3, B18, Bw35, Cw4, DRw2, Dw4

DNA Profile (STR): Amelogenin: X

CSF1PO: 10,12

D13S317: 12

D16S539: 9

T24細胞D5S818: 10,12

D7S820: 10,11

THO1: 6

TPOX: 8,11

vWA: 17

Cytogenetic Analysis: hypodiploidy to hypopentaploidy; stemline 86; 2 to 4 telocentrics; 3 to 4 minutes, hypotetraploid to hypertetraploid with abnormalities including dicentrics, breaks, pulverization, minutes and telocentric markers

Isoenzymes: AK-1, 1

ES-D, 1

G6PD, B

GLO-I, 1

Me-2, 1-2

PGM1, 1

PGM3, 1

Age: 81 years

Gender: female

Ethnicity: Caucasian

Comments: Leukocytes and sera from patients with transitional cell carcinoma were cytotoxic to T24 and related lines.

Cells have a 19 hour generation time.

Contains the ras (H-ras) oncogene.

Propagation: T24細胞ATCC complete growth medium: The base medium for this cell line is ATCC-formulated McCoy's 5a Medium Modified, Catalog No. 30-2007. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Temperature: 37.0°C

Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:8 is recommended

Medium Renewal: 2 to 3 times per week

Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.

Add fresh culture medium, aspirate and dispense into new culture flasks.

Preservation: Culture medium, 95%; DMSO, 5%

Related Products: recommended serum:ATCC 30-2020

References: 21849: O'Toole CHuman bladder cancer lines: HLA Class I and Class II antigen expression and susceptibility to cytostatic and cytotoxic effects in vitroIn: O'Toole CIn vitro models for cancer researchvol. IVBoca Raton, FLCRC Presspp. 103-125.

22365: O'Toole C, et al. Cellular immunity to human urinary bladder carcinoma. I. Correlation to clinical stage and radiotherapy. Int. J. Cancer 10: 77-91, 1972. PubMed: 4196436

22443: Williams BY, Schonbrunn A. Bombesin receptors in a human duodenal tumor cell line: binding properties and function. Cancer Res. 54: 818-824, 1994. PubMed: 8306345

22510: Bubenik J, et al. Cellular and humoral immune responses to human urinary bladder carcinomas. Int. J. Cancer 5: 310-319, 1970. PubMed: 5452065

22536: Fogh J, et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. PubMed: 833871

22539: Fogh J, et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 327080

22849: Bubenik J, et al. T24細胞Established cell line of urinary bladder carcinoma (T24) containing tumour-specific antigen. Int. J. Cancer 11: 765-773, 1973. PubMed: 4133950

23226: Pollack MS, et al. HLA-A, B, C and DR alloantigen expression on forty-six cultured human tumor cell lines. J. Natl. Cancer Inst. 66: 1003-1012, 1981. PubMed: 7017212

23256: Carey TE, et al. Cell surface antigens of human malignant melanoma: mixed hemadsorption assays for humoral immunity to cultured autologous melanoma cells. Proc. Natl. Acad. Sci. USA 73: 3278-3282, 1976. PubMed: 1067619

24381: Fogh J. Cultivation, characterization, and identification of human tumor cells with emphasis on kidney, testis, and bladder tumors. Natl. Cancer Inst. Monogr. 49: 5-9, 1978. PubMed: 571047

25065: Bellet D, et al. Malignant transformation of nontrophoblastic cells is associated with the expression of chorionic gonadotropin beta genes normally transcribed in trophoblastic cells. Cancer Res. 57: 516-523, 1997. PubMed: 9012484

26316: Bubenik J, et al. Cellular immunity to renal carcinomas in man. Int. J. Cancer 8: 503-513, 1971. PubMed: 5137312

32266: Bender CM, et al. Inhibition of DNA methylation by 5-Aza-2'-deoxycytidine suppresses the growth of human tumor cell lines. Cancer Res. 58: 95-101, 1998. PubMed: 9426064

33025: Ponton A, et al. The CD95 (APO-1/Fas) receptor activates NF-kappaB independently of its cytotoxic function. J. Biol. Chem. 271: 8991-8995, 1996. PubMed: 8621545