H9/HTLV-IIIB細(xì)胞
生長(zhǎng)狀態(tài)： 懸浮生長(zhǎng)
數(shù)量： 大量
ATCC Number： CRL-8543?
細(xì)胞類型： T**細(xì)胞
是否是腫瘤細(xì)胞： 1
物種來源： 人
運(yùn)輸方式： 凍存運(yùn)輸
細(xì)胞形態(tài)： **樣
Designations: H9/HTLV-IIIB
H9/HTLV-IIIB細(xì)胞Depositors: The United States of America
Biosafety Level: 2 [Cells Contain RETROVIRUS ]
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Homo sapiens
Morphology: lymphoblast
Source: Cell Type: T lymphocyte;
Cellular Products: Human T cell leukemia virus III (HTLV III); Human Immunodeficiency virus (HIV)
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments: H9/HTLV-IIIB細(xì)胞The cells produce HTLV III.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Medium Renewal: Every 2 to 3 days
H9/HTLV-IIIB細(xì)胞Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
References: 3536: Popovic M, et al. Serological detection of antibodies to HTLV-III in sera of patients with AIDS and pre-AIDS conditions. US Patent 4,520,113 dated May 28 1985
22938: Chang SY, et al. The origin of HIV-1 isolate HTLV-IIIB. Nature 363: 466-469, 1993. PubMed: 8502298
32754: Trkola A, et al. Human momoclonal antibody 2G12 defines a distinctive neutralization epitope on the gp120 glycoprotein of human immunodeficiency virus type 1. J. Virol. 70: 1100-1108, 1996. H9/HTLV-IIIB細(xì)胞PubMed: 8551569
