HT-1376細(xì)胞
細(xì)胞形態(tài)： 上皮樣
ATCC Number： CRL-1472?
運(yùn)輸方式： 凍存運(yùn)輸
相關(guān)**： 腫瘤
數(shù)量： 大量
年限： grade 3
器官來源： 膀胱
是否是腫瘤細(xì)胞： 1
物種來源： 人
生長(zhǎng)狀態(tài)： 貼壁生長(zhǎng)
HT-1376細(xì)胞Designations: HT-1376
Depositors: S Rasheed
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens
Morphology: epithelial
Source: Organ: urinary bladder
Tumor Stage: grade 3
Disease: carcinoma
Cellular Products: fibrinolytic activity; interferon
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. HT-1376細(xì)胞Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Applications: transfection host
Tumorigenic: Yes
Reverse Transcript: negative
DNA Profile (STR): Amelogenin: X
CSF1PO: 12
D13S317: 9,11
D16S539: 11,14
D5S818: 11,12
D7S820: 9,12
THO1: 7,10
TPOX: 8
vWA: 15,18
Cytogenetic Analysis: modal chromosome number = 104 to 121
Isoenzymes: HT-1376細(xì)胞G6PD, B
Age: 58 years
Gender: female
Ethnicity: Caucasian
Comments: The line does not contain detectable virus particles, herpesvirus antigens or retrovirus antigens.
The cells will grow in soft agar.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: Every 2 to 3 days
Remove medium, add fresh 0.25% trypsin, 0.02% EDTA solution, rinse and remove. Allow culture to sit at room temperature or 37C until cells detach (about 10 minutes), add fresh medium, aspirate and dispense into new flasks.
Related Products: purified DNA:ATCC CRL-1472D
References: 22538: Rasheed S, et al. HT-1376細(xì)胞Human bladder carcinoma: characterization of two new tumor cell lines and search for tumor viruses. J. Natl. Cancer Inst. 58: 881-890, 1977. PubMed: 191628
23134: Jameson P, Grossberg SE. Production of interferon by human tumor cell lines. Arch. Virol. 62: 209-219, 1979. PubMed: 229805
25065: Bellet D, et al. Malignant transformation of nontrophoblastic cells is associated with the expression of chorionic gonadotropin beta genes normally transcribed in trophoblastic cells. Cancer Res. 57: 516-523, 1997. PubMed: 9012484
