Ramos.2G6.4C10細胞
運輸方式： 凍存運輸
細胞類型： B**細胞
是否是腫瘤細胞： 1
物種來源： 人
生長狀態： 懸浮生長
ATCC Number： CRL-1923?
相關**： 其他**
細胞形態： **樣
**類型： IgM
年限： 3 years
數量： 大量
Designations: Ramos.2G6.4C10
Depositors: JP Siegel
Isotype: IgM
Ramos.2G6.4C10細胞Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Homo sapiens
Morphology: lymphoblast
Source: Disease: Burkitt's lymphoma (American)
Cell Type: B lymphocyte;
Cellular Products: immunoglobulin (surface and secreted)
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Receptors: interleukin 4 (IL-4); low affinity IgE (CD23)
Antigen Expression: CD23 +
DNA Profile (STR): Amelogenin: X
CSF1PO: 10,11
D13S317: 13
D16S539: 10,13
Ramos.2G6.4C10細胞D5S818: 7,12
D7S820: 11
THO1: 7,9.3
TPOX: 8,9
vWA: 15,16
Age: 3 years
Gender: male
Ethnicity: Caucasian
Comments: This line was derived from the Ramos (RA-1) cell line (see ATCC CRL-1596) by repetitive cloning with selection for low constitutive expression of CD23 and maximum stimulation of CD23 expression by IL-4.
Both the parental line (ATCC CRL-1596)and the derivative cell line Ramos.2G6.4C10 (ATCC CRL-1923) have about 1500 IL-4 binding sites per cell. [23389]
Ramos.2G6.4C10 cells provide a sensitive and specific assay system for IL-4 that is insensitive to the presence of other lymphokines.
And cytokines such as interleukins (IL-2, IL-2 and IL-6), colony stimulating factors (G-CSF, M-CSF and GM-CSF) and interferons alpha and gamma (IFN alpha, IFN gamma).
Tumor necrosis factors alpha and beta (TNF alpha, TNF beta) cause only minor interference.
The assay is sensitive to IL-4 concentrations in the range 50 to 800 pg/ml.
Propagation: Ramos.2G6.4C10細胞ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Protocol: Cultures can be maintained by addition or replacement of fresh medium. Establish new cultures at 5 X 10 exp5 viable cells/ml and maintain between 1 and 2 X 10 exp6 cells/ml.
Medium Renewal: Add fresh medium every 4 to 6 days (depending on cell density)
Preservation: Freeze medium: Complete growth medium, 92.5%; DMSO, 7.5%
Storage temperature: liquid nitrogen vapor temperature
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001
recommended serum:ATCC 30-2020
parental cell line:ATCC CRL-1596
References: 22747: Klein G, et al. An EBV-genome-negative cell line established from an American Burkitt lymphoma; receptor characteristics. EBV infectibility and permanent conversion into EBV-positive sublines by in vitro infection. Intervirology 5: 319-334, 1975. PubMed: 181343
23389: Siegel JP, Mostowski HS. A bioassay for the measurement of human interleukin-4. J. Immunol. Methods 132: 287-295, 1990. PubMed: 1698879
23468: Rousset F, et al. Regulation of Fc receptor for IgE (CD23) and class II MHC antigen expression on Burkitt's lymphoma cell lines by human IL-4 and IFN-gamma. J. Immunol. 140: 2625-2632, 1988. Ramos.2G6.4C10細胞PubMed: 2965726
