HCC1954細胞
器官來源： **
組織來源： duct
生長狀態： 貼壁生長
數量： 大量
運輸方式： 凍存運輸
年限： TNM stage IIA, grade 3
是否是腫瘤細胞： 1
物種來源： 人
ATCC Number： CRL-2338?
相關**： 導管癌
細胞形態： 其他
Designations: HCC1954
Depositors: AF Gazdar, AK Virmani
HCC1954細胞Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens
Morphology: large epithelial cells with occasional vacuoles
Source: Organ: mammary gland; breast
Tissue: duct
Tumor Stage: TNM stage IIA, grade 3
Disease: ductal carcinoma
Cellular Products: Epithelial glycoprotein 2 [EGP2]; cytokeratin 19
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Restrictions: The line is available with the following restrictions: 1. This cell line was deposited at the ATCC by Dr. Adi F. Gazdar and is provided for research purposes only. Neither the cell line nor products derived from it may be sold or used for commercial purposes. Nor can the cells be distributed to third parties for purposes of sale, or producing for sale, cells or their products. The cells are provided as service to the research community. HCC1954細胞They are provided without warranty of merchantability or fitness for a particular purpose or any other warranty, expressed or implied. 2. Any proposed commercial use of the these cells, or their products must first be negotiated with the University of Texas Southwestern Medical Center at Dallas, 5323 Harry Hines Blvd., Dallas, Texas 75235. Telephone (214) 648-1888, Email TechnologyDevelopment@UTSouthwestern.edu, or Fax: (214) 951-0935.
Isolation: Isolation date: October 30, 1995
Applications: HCC1954 is positive for the epithelial cell specific marker Epithelial Glycoprotein 2 and for cytokeratin 19, and is negative for expression of estrogen receptor (ER) and progesterone receptor (PR).
HCC1954 was derived from a primary stage IIA, grade 3 invasive ductal carcinoma with no lymph node metastases.
Receptors: estrogen receptor, not expressed
progesterone receptor, not expressed
Oncogene: her2/neu + (overexpressed)
DNA Profile (STR): Amelogenin: X
CSF1PO: 10
D13S317: 8,9
D16S539: 9,11
D5S818: 11
D7S820: 10,11
THO1: 6,7
TPOX: 8,9
vWA: 18,19
HCC1954細胞Age: 61 years *****
Gender: female
Ethnicity: East Indian
Comments: The HCC1954 is a poorly differentiated cell line initiated on October 30, 1995; it took about 4 months to establish.
HCC1954 was derived from a primary stage IIA, grade 3 invasive ductal carcinoma with no lymph node metastases.
HCC1954 is positive for the epithelial cell specific marker Epithelial Glycoprotein 2 and for cytokeratin 19, and is negative for expression of estrogen receptor (ER) and progesterone receptor (PR).
Her2/neu is overexpressed in the ELISA assay.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Protocol:
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37?C to facilitate dispersal.
Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37?C.
Subcultivation Ratio: HCC1954細胞A subcultivation ratio of 1:4 to 1:8 is recommended
Medium Renewal: Every 2 to 3 days
Preservation: Freeze medium: Complete growth medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001
recommended serum:ATCC 30-2020
normal (or near-normal) cell line established from the same patient:ATCC CRL-2339
purified DNA:ATCC CRL-2338D
purified RNA:ATCC CRL-2338R
cell pellet:ATCC CRL-2338P
References: 38266: Gazdar AF, et al. Characterization of paired tumor and non-tumor cell lines established from patients with breast cancer. Int. J. Cancer 78: 766-774, 1998. PubMed: 9833771
