ST486細胞
數(shù)量： 大量
ATCC Number： CRL-1647?
相關**： **瘤
細胞類型： B**細胞
是否是腫瘤細胞： 1
物種來源： 人
生長狀態(tài)： 懸浮生長
運輸方式： 凍存運輸
細胞形態(tài)： **樣
組織來源： ascites
Designations: ST486
Depositors: IT Magrath
ST486細胞Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Homo sapiens
Morphology: lymphoblast
Source: Tissue: ascites
Disease: Burkitt's lymphoma
Cell Type: B lymphocyte;
Cellular Products: IgM; kappa light chain
immunoglobulin (surface and secreted)
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Receptors: ST486細胞complement
Antigen Expression: HLA A2, A10 (Aw25), B5 (Bw52), B18
DNA Profile (STR): Amelogenin: X
CSF1PO: 10,12
D13S317: 8,12
D16S539: 9,12
D5S818: 11,12
D7S820: 7,12
THO1: 7,9
TPOX: 8,11
vWA: 17,18
Gender: female
Comments: The cells are EBNA negative and are negative for Fc receptors.
Approximately 2% of the cells express commplement receptors.
Propagation: ST486細胞ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing: Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 to 3 X 10 exp5 viable cells/ml.Maintain cell density between 2 X 10 exp5 and 2 X 10 exp6 viable cells/ml.
Medium Renewal: Every 2 to 3 days
Preservation: Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Doubling Time: 19 to 20 hrs
References: 1093: Benjamin D, et al. Immunoglobulin secretion by cell lines derived from African and American undifferentiated lymphomas of Burkitt's and non-Burkitt's type. J. Immunol. 129: 1336-1342, 1982. PubMed: 6286763
22548: Magrath IT, et al. Characterization of lymphoma-derived cell lines: comparison of cell lines positive and negative for Epstein-Barr virus nuclear antigen. I. Physical, cytogenetic, and growth characteristics. J. Natl. Cancer Inst. 64: 465-476, 1980. PubMed: 6243721
22549: Magrath IT, et al. Characterization of lymphoma-derived cell lines: comparison of cell lines positive and negative for Epstein-Barr virus nuclear antigen. II. Surface markers. J. Natl. ST486細胞Cancer Inst. 64: 477-483, 1980. PubMed: 6243722
