CHL-1細胞

數量： 大量

器官來源： 皮膚

運輸方式： 凍存運輸

細胞形態： 上皮樣

ATCC Number： CRL-9446?

相關**： 黑色素瘤

生長狀態： 貼壁生長

是否是腫瘤細胞： 1

物種來源： 人

Designations: CHL-1

CHL-1細胞Depositors: Berlex Laboratories, Inc.

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens

Morphology: epithelial

Source: Organ: skin

Disease: melanoma

Cellular Products: human tissue plasminogen activator (t-PA)

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Applications: CHL-1細胞transfection host

Transfection host derived from RPMI 7932 that can grow to a higher density, namely 5 x 10(7) cells/ml. [41429]

DNA Profile (STR): Amelogenin: X

CSF1PO: 10

D13S317: 8,11

D16S539: 13

D5S818: 11,12

D7S820: 11,13

THO1: 9,9.3

TPOX: 8

vWA: 17

Comments: This is a derivative of the RPMI 7932 human melanoma cell line (Bowes).

It produces about 0.1 mU of t-PA per cell per day.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. CHL-1細胞To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Temperature: 37.0°C

Subculturing: Protocol: Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.

Subcultivation Ratio: A subcultivation ratio of 1:6 to 1:10 is recommended

Medium Renewal: Add fresh medium at the time of subculture

Preservation: Freeze medium: Complete growth medium, 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor temperature

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002

recommended serum:ATCC 30-2020

References: 41429: Cashion L, et al. CHL-1細胞Transfected cells containing plasmids having genes oriented in opposing directions and methods of using. US Patent 5,017,478 dated May 21 1991