M059J細(xì)胞
細(xì)胞類型： 其他細(xì)胞類型
是否是腫瘤細(xì)胞： 1
物種來源： 人
數(shù)量： 大量
年限： 33 years
器官來源： 大腦
生長狀態(tài)： 貼壁生長
ATCC Number： CRL-2366?
相關(guān)**： 其他**
細(xì)胞形態(tài)： 成纖維樣
運輸方式： 凍存運輸
Designations: M059J
M059J細(xì)胞Depositors: J Allalunis-Turner, RS Day
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens
Morphology: fibroblast
Source: Organ: brain
Disease: malignant glioblastoma; glioma
Cell Type: glial cell;
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
DNA Profile (STR): Amelogenin: X,Y
CSF1PO: 10,12
D13S317: 14
D16S539: 10,12
D5S818: 11,12
D7S820: 10,12
M059J細(xì)胞THO1: 9.3
TPOX: 8
vWA: 17
Cytogenetic Analysis: aneuploid; Y chromosome is present
Age: 33 years
Gender: male
Comments: The cells were isolated concurrently from the same tumor specimen as M059K (see CRL-2365).
M059J cells lack DNA-dependent protein kinase activity, while M059K cells express normal levels of DNA-dependent protein kinase
M059J cells are approximately 30-fold more sensitive to ionizing radiation than M059K cells
M059J cells are more sensitive than M059K cells to the cytotoxic effects of bleomycin, N,N-bis(2-choroethyl)-N-nitrosourea and nitrogen mustard
M059J cells are deficient in repair of DNA double strand breaks
The cells are negative for glial fibrillary acidic protein (GFAP)
Together, M059K and M059J provide a useful model system in which to study the role of DNA protein kinase in cellular and molecular processes involving DNA damage recognition and repair
M059J cells were isolated from a tumor specimen taken from a 33 year old male with untreated malignant glioblastoma
Propagation: M059J細(xì)胞ATCC complete growth medium: These cells are grown in a medium containing a 1:1 mixture of Dulbecco's Modified Eagle's Medium and Ham's F12 medium with 2.5 mM L-glutamine adjusted to contain 15 mM HEPES, 0.5 mM sodium pyruvate, and 1.2 g/L sodium bicarbonate supplemented with 0.05 mM non-essential amino acids and 10% fetal bovine serum.
Temperature: 37.0°C
Subculturing: Protocol: Remove medium, and rinse with 0.25% trypsin, 0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:6 to 1:8 is recommended
Medium Renewal: Every 2 to 3 days
Preservation: Freeze medium: Complete growth medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor temperature
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2006
recommended serum:ATCC 30-2020
derived from same individual:ATCC CRL-2365
References: 33940: Allalunis-Turner MJ, et al. Isolation of two lines from a human malignant glioma specimen differing in sensitivity to radiation and chemotherapeutic drugs. Radiat. Res. 134: 349-354, 1993. PubMed: 8316628
33942: Lees-Miller SP, et al. M059J細(xì)胞Absence of p350 subunit of DNA activated protein kinase from a radiosensitive human cell line. Science 267: 1183-1185, 1995. PubMed: 7855602
38596: Allalunis-Turner J, et al. Intact G2-phase checkpoint in cells of a human cell line lacking DNA-dependent protein kinase activity. Radiat. Res. 147: 284-287, 1997. PubMed: 9052673
38598: Allalunis-Turner MJ, et al. Radiation-induced DNA damage and repair in cells of a radiosensitive human malignant glioma cell line. Radiat. Res. 144: 288-293, 1995. PubMed: 7494872
38599: Wang J, et al. Radiation-induced damage in two human glioma cell lines as measured by the nucleoid assay. Anticancer Res. 17: 4615-4618, 1997. PubMed: 9494578
