RD-ES細(xì)胞
數(shù)量： 大量
是否是腫瘤細(xì)胞： 1
物種來源： 人
ATCC Number： HTB-166?
相關(guān)**： 其他**
細(xì)胞形態(tài)： 上皮樣
生長(zhǎng)狀態(tài)： 混合型生長(zhǎng)
運(yùn)輸方式： 凍存運(yùn)輸
年限： 19 years
器官來源： 骨
Designations: RD-ES
RD-ES細(xì)胞Depositors: G Marshall, M Kirchen
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: mixed: adherent and clusters in suspension
Organism: Homo sapiens
Morphology: epithelial
Source: Organ: bone
Disease: Ewing's sarcoma
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Antigen Expression: Blood type B; Rh+
Isoenzymes: AK-1, 1
ES-D, 1
G6PD, B
GLO-I, 1-2
Me-2, 1-2
RD-ES細(xì)胞PGM1, 1-2
PGM3, 1
Age: 19 years
Gender: male
Ethnicity: Caucasian
Comments: The cell line was initiated by G. Marshall and M. Kirchen from a primary osseous Ewings sarcoma of the humerus.
Ultrastructurally the cells exhibit primitive cell junctions, possess glycogen pools and are 20 to 25 microns in diameter.
The cells grow as a loosely attached monolayer in small clusters of 5 to 10 cells.
Propagation: RD-ES細(xì)胞ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 15%.
Temperature: 37.0°C
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:8 is recommended
Medium Renewal: 2 to 3 times per week
Shake the flask after removing most of the medium. Add fresh medium and transfer to new flasks.
Preservation: culture medium 95%; DMSO, 5%
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001
recommended serum:RD-ES細(xì)胞ATCC 30-2020
