CAMA-1細(xì)胞
是否是腫瘤細(xì)胞： 1
物種來(lái)源： 人
數(shù)量： 大量
細(xì)胞形態(tài)： 上皮樣
器官來(lái)源： **
生長(zhǎng)狀態(tài)： 貼壁生長(zhǎng)
年限： 51 years
ATCC Number： HTB-21?
相關(guān)**： 腺癌
運(yùn)輸方式： 凍存運(yùn)輸
Designations: CAMA-1
Depositors: J Fogh
CAMA-1細(xì)胞Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: Adherent patches of epithelial cells; compact, multilayered colonies, rarely become confluent
Organism: Homo sapiens
Morphology: epithelial
Source: Organ: mammary gland; breast
Disease: adenocarcinoma
Derived from metastatic site: pleural effusion
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Restrictions: The cells are distributed for research purposes only. The Memorial Sloan-Kettering Cancer Center releases the line subject to the following: 1.) CAMA-1細(xì)胞The cells or their products must not be distributed to third parties. Commercial interests are the exclusive property of Memorial Sloan-Kettering Cancer Center. 2.) Any proposed commercial use of these cells must first be negotiated with The Director, Office of Industrial Affairs, Memorial Sloan-Kettering Cancer Center, 1275 York Avenue, New York, NY 10021; phone (212) 639-6181; FAX (212) 717-3439.
Isolation: Isolation date: 1975
Tumorigenic: Yes
Antigen Expression: Blood type O; Rh +; HLA A10, A11, B12, B18
DNA Profile (STR): Amelogenin: X
CSF1PO: 10,12
D13S317: 12
D16S539: 11
D5S818: 12,13
D7S820: 8,11
THO1: 8,9.3
TPOX: 8
vWA: 15
Cytogenetic Analysis: modal number = 80; range = 68 to 83.
CAMA-1細(xì)胞This is a hypertriploid human cell line with the modal chromosome number of 80 occurring in 44% of a total of 88 cells examined. The rate of polyploid cells was 3.5%. Karyotypes of this cell line were generally uniform and stable. There were 12-13 marker chromosomes per cell, 11 of which were found in all cells, and 7 of which were mostly paired. Among the markers were paired mar(1qter--q21::?::6p11.2--6pter), t(3q::?), and i(16q); single der(12)t(12;?)(q24:?); and 8-9 others. Double minutes occurred in some cells; however, they were present as only one or two copies per cell. Structurally normal N16 was absent and N8 occurred in only a few cells. Paired normal X chromosomes were present in every cell.
Isoenzymes: AK-1, 1
ES-D, 1-2
G6PD, B
GLO-I, 1-2
Me-2, 1
PGM1, 1
PGM3, 1
Age: 51 years
Gender: female
Ethnicity: Caucasian
Comments: The CAMA-1 line was established by J. Fogh at Sloan-Kettering in 1975 from cells in the pleural effusion of a patient with carcinoma of the breast.
An ampule frozen in May of 1978 at passage 21 was provided to the ATCC in June of 1991.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. CAMA-1細(xì)胞To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Subculturing: Protocol:
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37?C to facilitate dispersal.
Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37?C.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:4 is recommended
Medium Renewal: 2 to 3 times per week
Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003
recommended serum:ATCC 30-2020
References: 22536: Fogh J, et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. PubMed: 833871
