D341 Med細(xì)胞
是否是腫瘤細(xì)胞： 1
物種來(lái)源： 人
組織來(lái)源： cerebellum
器官來(lái)源： 大腦
ATCC Number： HTB-187?
運(yùn)輸方式： 凍存運(yùn)輸
相關(guān)**： 其他**
數(shù)量： 大量
細(xì)胞形態(tài)： 其他
生長(zhǎng)狀態(tài)： 懸浮生長(zhǎng)
年限： 3.5 years
Designations: D341 Med
D341 Med細(xì)胞Depositors: HS Friedman
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Homo sapiens
Morphology: spheroid
Source: Organ: brain
Tissue: cerebellum
Disease: medulloblastoma
Cellular Products: neurofilament proteins
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Tumorigenic: Yes
DNA Profile (STR): Amelogenin: X,Y
CSF1PO: 10,11
D13S317: 11,13
D16S539: 12,14
D5S818: 11,12
D341 Med細(xì)胞D7S820: 9,13
F13A01: 6,7
F13B: 8,10
FESFPS: 10,13
LPL: 11
THO1: 6,9.3
TPOX: 8,11
vWA: 17,18
Cytogenetic Analysis: This is a hyperdiploid (modal number = 49; range = 44 to 50) human cell line with higher ploidies occurring with a frequency of 8.5%. Four marker chromosomes are present in all cells. They are: 11HSR, i(17q), der(22)t(1;22)(q12;p12) and 8q-. There are three copies each of N6 and N18, and single copies each of N11, N17 and N22. The single X chromosome was structurally normal, and the presence of the Y chromosome was confirmed by fluorescence microsopy.
Age: 3.5 years
Gender: male
Comments: The D341 Med cell line was established in 1988 by Friedman et al. from tumor tissue taken from a boy with medulloblastoma.
The cells produce tumors in nude mice.
The cell line is positive for expression of neurofibrillary proteins, glutamine synthetase and neuron specific enolase but negative for glial fibrillary acidic proteins and S100 (S-100) protein.
The cells are positive for the neuroectodermal antigen recognized by the UJ13A monoclonal antibody.
The c-myc oncogene is amplified in D341 Med cells.
Propagation:D341 Med細(xì)胞 ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%.
Temperature: 37.0°C
Subculturing: Protocol: Cultures can be maintained by addition or replacement of medium. Adherent cells can be dislodged by scraping, and cultures can be established by resuspending a cell pellet at 5 X 10(5) viable cells/ml. Maintain cultures between 4 X 10(5) and 1 X 10(6) cells/ml.
Medium Renewal: 2 to 3 times per week
Preservation: Freeze medium: culture medium, 90%; DMSO, 10%
Storage temperature: liquid nitrogen vapor phase
Doubling Time: 37 hrs
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003
recommended serum:ATCC 30-2020
References: 2096: Friedman HS, et al. Phenotypic and genotypic analysis of a human meduloblastoma cell line and transplantable xenograft (D341 Med) demonstrating amplification of c-myc. Am. J. Pathol. 130: 472-484, 1988. PubMed: 3279793
23092: He XM, et al. Expression of O6-methylguanine-DNA methyltransferase in six human medulloblastoma cell lines. Cancer Res. 52: 1144-1148, 1992. PubMed: 1737373
32287: Rostomily RC, et al. D341 Med細(xì)胞Expression of neurogenic basic helix-loop-helix genes in primitive neuroectodermal tumors. Cancer Res. 57: 3526-3531, 1997. PubMed: 9270024
