ES-2細(xì)胞
是否是腫瘤細(xì)胞： 1
物種來源： 人
年限： 47 years
運(yùn)輸方式： 凍存運(yùn)輸
細(xì)胞形態(tài)： 成纖維樣
生長(zhǎng)狀態(tài)： 貼壁生長(zhǎng)
數(shù)量： 大量
器官來源： 卵巢
ATCC Number： CRL-1978?
相關(guān)**： 其他**
Designations: ES-2
Depositors: BI Sikic
ES-2細(xì)胞Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens
Morphology: fibroblast
Source: Organ: ovary
Disease: clear cell carcinoma
Cellular Products: P glycoprotein
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Tumorigenic: Yes
DNA Profile (STR): Amelogenin: X
CSF1PO: 10,15
D13S317: 11
D16S539: 11,13
D5S818: 11,13
D7S820: 11
THO1: 9.3
TPOX: 8,12
vWA: 16,17
Cytogenetic Analysis: ES-2細(xì)胞This line has a complex hyperdiploid karyotype of 66XX to 88XX.
Age: 47 years
Gender: female
Ethnicity: Black
Comments: The ES-2 cell line was established from a surgical tumor specimen taken from a 47 year old black woman.
The tumor was described as a poorly differentiated ovarian clear cell carcinoma.
Initially, the cells were grown in soft agar.
The cells exhibit low to moderate resistance to a number of chemotherapeutic agents including doxorubicin, cisplatin, carmustine, etoposide and cyanomorpholinodoxorubicin (MRA-CN).
ES-2 cells express low levels of P glycoprotein.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated McCoy's 5a Medium Modified, Catalog No. 30-2007. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Protocol: Remove spent medium, add fresh EDTA solution (0.15 g disodium EDTA, 4.0 g NaCL, 0.28 g sodium bicarbonate, 0.5 g dextrose and 0.2 g KCl dissolved in 500 ml double distilled water). Allow the cells to sit at room temperature for a few minutes, and dislodge the cells by rapping the side of the flask sharply with the palm of your hand. Add fresh medium, aspirate and dispense into new flasks.
Subcultivation Ratio: ES-2細(xì)胞A subcultivation ratio of 1:4 to 1:8 is recommended
Medium Renewal: 2 to 3 times per week
Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Doubling Time: 24 hrs
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2007
recommended serum:ATCC 30-2020
References: 23089: Lau DH, et al. Multifactorial mechanisms associated with broad cross-resistance of ovarian carcinoma cells selected by cyanomorpholino doxorubicin. Cancer Res. 51: 5181-5187, 1991. ES-2細(xì)胞PubMed: 1717140
