SW 156細胞
器官來源： 腎臟
細胞形態(tài)： 上皮樣
生長狀態(tài)： 貼壁生長
運輸方式： 凍存運輸
數(shù)量： 大量
是否是腫瘤細胞： 1
物種來源： 人
ATCC Number： CRL-2175?
相關(guān)**： 其他**
SW 156細胞年限： 75 years
Designations: SW 156 [SW-156, SW156]
Depositors: W McCombs
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens
Morphology: epithelial
Source: Organ: kidney
Disease: hypernephroma
Permits/Forms: SW 156細胞In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Restrictions: These cells are distributed for research purposes only. The Scott and White Clinic releases the line subject to the following: 1) The cells or products derived from them must not be sold or used for commercial purposes. Nor can the cells be distributed to third parties for purpose of sale, or producing for sale, cells or their products. Commercial interests are the exclusive property of the Scott and White Clinic. 2) Any proposed commercial use of these cells or products produced by them must first be negotiated with the Scott and White Clinic, 2401 S. 31 Street, Temple, Texas 76508. Telephone (817) 774-2432
Antigen Expression: Blood Type O; Rh +
Age: 75 years
Gender: male
Ethnicity: Caucasian
Comments: The SW 156 line was established in 1972 by A. Leibovitz from a hypernephroma.
SW 156細胞Propagation: ATCC complete growth medium: Leibovitz's L-15 medium with 2mM L-glutamine, 90%; fetal bovine serum, 10%
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
Remove spent medium, add fresh 0.25% trypsin, 0.03% EDTA solution, rinse and remove trypsin.
Add fresh trypsin solution (1 to 2 ml), and let the culture sit at room temperature (or at 37C) until the cells detach.
Add fresh medium, aspirate and dispense into new flasks.
Preservation: Culture medium, 95%; DMSO, 5%
References: 22536: Fogh J, et al. SW 156細胞Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. PubMed: 833871
24381: Fogh J. Cultivation, characterization, and identification of human tumor cells with emphasis on kidney, testis, and bladder tumors. Natl. Cancer Inst. Monogr. 49: 5-9, 1978. PubMed: 571047
