AS 33細胞
是否是腫瘤細胞： 0
物種來源： 小鼠
ATCC Number： HB-8779?
運輸方式： 凍存運輸
生長狀態(tài)： 懸浮生長
細胞形態(tài)： **樣
**類型： IgG2a , IgG2a
數(shù)量： 大量
Designations: AS 33
AS 33細胞Depositors: Sloan-Kettering Institute for Cancer Research
Isotype: IgG2a , IgG2a
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products: immunoglobulin; monoclonal antibody; against a heat stable human carcinoma associated antigen
Permits/Forms: AS 33細胞In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Restrictions: The cells are distributed for research purposes only. The Memorial Sloan-Kettering Cancer Center releases the line subject to the following: 1.) The cells or their products must not be distributed to third parties. Commercial interests are the exclusive property of Memorial Sloan-Kettering Cancer Center. 2.) Any proposed commercial use of these cells must first be negotiated with The Director, Office of Industrial Affairs, Memorial Sloan-Kettering Cancer Center, 1275 York Avenue, New York, NY 10021; phone (212) 639-6181; FAX (212) 717-3439.
Tumorigenic: Yes
Comments: Animals were immunized with the ASPC-1 human pancreatic carcinoma cell line.
Spleen cells were fused with NS-1 myeloma cells.
The antibody reacts with some colon and pancreatic carcinoma cell lines as well as some T cell leukemia lines.
AS 33細胞In tissues, the reactivity is restricted to carcinomas and normal colonic mucosa.
Some reactivity was also detected in salivary gland and breast tissue.
Other normal tissues test were negative.
The AS 33 antigen is heat stable.
Propagation: ATCC complete growth medium: RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate and supplemented with 0.1 mM hypoxanthine and 0.016 mM thymidine, 90%; fetal bovine serum, 10%
Temperature: 37.0°C
Growth Conditions: These cells must be grown using a feeder layer. Mouse peritoneal exudate cells or irradiated mouse fibroblasts (e.g., irradiated STO cells, ATCC 56-X) will provide suitable feeder layers.
Subculturing: Protocol: Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10(5) cells/ml and maintain between 1 X 10(5) and 1 X 10(6) cells/ml.
Medium Renewal: Every 2 to 3 days
Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature:AS 33細胞liquid nitrogen vapor phase
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001
recommended serum:ATCC 30-2020
feeder layer cells:ATCC 56-X
References: 22006: Welt S, et al. Method of imaging colorectal carcinoma lesion and composition for use therein. US Patent 5,160,723 dated Nov 3 1992
45098: Welt S, et al. Methods of treating colon cancer utilizing tumor-specific antibodies. US Patent 5,851,526 dated Dec 22 1998
