TE16細胞
細胞形態(tài)： **樣
**類型： IgG1
ATCC Number： HB-210?
數(shù)量： 大量
是否是腫瘤細胞： 0
物種來源： 小鼠
生長狀態(tài)： 懸浮生長
運輸方式： 凍存運輸
Designations: TE16
TE16細胞Depositors: BF Haynes
Isotype: IgG1
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products: TE16細胞immunoglobulin; monoclonal antibody; against human thymic epithelium (Hassall's bodies)
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Comments: Animals were immunized with human thymic stroma.
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
The antibody reacts with cells forming the outer swirls of Hassall's bodies.
TE16細胞It defines an antigen that is acquired between 14 and 16 weeks of fetal gestation, and marks the terminal stages of epidermal keratinocyte maturation.
The antibody binds specifically to cells forming the outer swirls of Hassall's bodies and also to stratum granulosum of skin epidermis and foreskin.
Tested and found negative for ectromelia virus (mousepox).
Propagation: ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 80%; heat-inactivated fetal bovine serum, 20%
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
References: 1637: Lobach DF, et al. TE16細胞The human thymic microenvironment. Phenotypic characterization of Hassall's bodies with the use of monoclonal antibodies. J. Immunol. 134: 250-257, 1985. PubMed: 2578044
