1D11.16.8細(xì)胞
ATCC Number： HB-9849?
細(xì)胞形態(tài)： **樣
**類型： IgG1 , IgG1
生長(zhǎng)狀態(tài)： 懸浮生長(zhǎng)
運(yùn)輸方式： 凍存運(yùn)輸
是否是腫瘤細(xì)胞： 0
物種來(lái)源： 小鼠
數(shù)量： 大量
Designations: 1D11.16.8
1D11.16.8細(xì)胞Depositors: Celtrix Pharmaceuticals, Inc.
Isotype: IgG1 , IgG1
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products: 1D11.16.8細(xì)胞immunoglobulin; monoclonal antibody; against transforming growth factor beta2 (TGF-beta2)
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Tumorigenic: Yes
Comments: Animals were immunized with a bovine TGF-beta2.
Spleen cells were fused with Sp2/0 myeloma cells.
The antibody reacts with both TGF-beta1 and TGF-beta2, and can inhibit their biological activities.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium:
O.1 mM Non-Essential Amino Acids (NEAA)
fetal bovine serum to a final concentration of 10%
1D11.16.8細(xì)胞Temperature: 37.0°C
Subculturing: Protocol: Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Medium Renewal: Every 2 to 3 days
Preservation: Freeze medium: Complete growth medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2020
References: 22122: Dasch JR, et al. Monoclonal antibodies which bind both transforming growth factors .beta.1 and .beta.2 and methods of use. US Patent 5,571,714 dated Nov 5 1996
39037: Dasch JR, et al. 1D11.16.8細(xì)胞Monoclonal antibodies to transforming growth factor-beta and methods of use. US Patent 5,772,998 dated Jun 30 1998
39038: Dasch JR, et al. Monoclonal antibodies to transforming growth factor-beta and methods of use. US Patent 5,783,185 dated Jul 21 1998
