LL 29 (AnHa)細胞
數量： 大量
細胞形態： 成纖維樣
生長狀態： 貼壁生長
年限： 26 years
ATCC Number： CCL-134?
相關**： 其他**
細胞類型： 成纖維細胞
LL 29 (AnHa)細胞是否是腫瘤細胞： 0
物種來源： 人
運輸方式： 凍存運輸
器官來源： 肺
Designations: LL 29 (AnHa)
Depositors: RG Crystal
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: LL 29 (AnHa)細胞Homo sapiens
Morphology: fibroblast
Source: Organ: lung
Disease: idiopathic pulmonary fibrosis
Cell Type: fibroblast
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Age: 26 years
LL 29 (AnHa)細胞Gender: female
Ethnicity: Caucasian
Propagation: ATCC complete growth medium: Ham's F12K medium, 85%; fetal bovine serum, 15%
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended
Medium Renewal: 2 to 3 times per week
Remove medium and add fresh 0.25% trypsin, 0.03% EDTA for 1 to 2 minutes. Remove trypsin, EDTA and incubate the culture at 37C for 10 to 20 minutes with a small volume of trypsin - EDTA. LL 29 (AnHa)細胞Add fresh medium, aspirate and dispense into new flasks.
Preservation: Culture medium, 95%; DMSO, 5%
