12MBr6細胞
器官來源： 肺
組織來源： bronchus
運輸方式： 凍存運輸
細胞形態： 上皮樣
生長狀態： 貼壁生長
ATCC Number： CRL-1576?
相關**： 正常
年限： 2 to 3 years
細胞類型： 上皮細胞
數量： 大量
12MBr6細胞是否是腫瘤細胞： 0
物種來源： 綠長尾猴
Designations: 12MBr6
Depositors: JL Caputo
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Cercopithecus aethiops
Morphology: epithelial
Source: Organ: lung
Tissue: bronchus
Disease: normal
Cell Type: 12MBr6細胞epithelial
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Isolation: Isolation date: July, 1979
Applications: Epidermal growth factor (EGF) was used to initiate and maintain growth.
Post-freeze longevity studies indicate that EGF is an essential component of the culture medium for successful long-term propagation of the cells.
12MBr6 is an epithelial cell line derived from secondary bronchial tissue of a 2- to 3-year-old normal African green monkey.
Receptors: epidermal growth factor (EGF)
Cytogenetic Analysis: modal number = 62; range = 43 to 64.
The modal chromosome number was found in 78% of the cell population. The rate of polyploidy was 2.4%. Three consistent chromosome markers were identified: M1: del(1)9 ?; M2 del(13) and M3. Chromosome #20 is trisomic and the X chromosome is found in the disomic condition.
Age: 2 to 3 years
12MBr6細胞Gender: male
Comments: 12MBr6 is an epithelial cell line derived from secondary bronchial tissue of a 2- to 3-year-old normal African green monkey. This line was initiated in June, 1979 at the American Type Culture Collection from tissue explants using penicylinders and a trypsin-versene solution for cell dispersal. These cells contain PAS-positive cytoplasmic inclusions. Epidermal growth factor (EGF) was used to initiate and maintain growth. Post-freeze longevity studies indicate that EGF is an essential component of the culture medium for successful long-term propagation of the cells. Progeny from the 12MBr6 were found to undergo at least 23 additional doublings beyond the frozen state.
Propagation: ATCC complete growth medium: Ham's F-12K Medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and supplemented with 30ng/ml epidermal growth factor and 10% fetal bovine serum
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Growth Conditions: The line requires epidermal growth factor as a supplement to the medium.
Subculturing: Protocol: .
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: 12MBr6細胞To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37?C to facilitate dispersal.
Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.Add fresh medium, aspirate and dispense into new flasks using a minimum inoculum of 6 X 10(4) cells/sq cm.
Incubate cultures at 37?C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: 2 to 3 times weekly
Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2004
recommended serum:ATCC 30-2020
