LS129-3C3-E3-1 [3C3]細胞

是否是腫瘤細胞： 0

物種來源： 小鼠

ATCC Number： HB-12653?

運輸方式： 凍存運輸

細胞形態： **樣

**類型： IgG2b

生長狀態： 懸浮生長

數量： 大量

LS129-3C3-E3-1 [3C3]細胞Designations: LS129-3C3-E3-1 [3C3]

Depositors: LeukoSite, Inc.

Isotype: IgG2b

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Mus musculus (B cell); Mus musculus (lymphoma)

LS129-3C3-E3-1 [3C3]細胞Morphology: lymphoblast

Source: Cell Type: hybridoma:lymphoblast B lymphocyte;

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Comments: Animals were immunized with a peptide consisting of the NH2 terminus of GPR-9-6 having the sequence MADDYGSESTSSMEDYVNFNFTDFYC.Spleen cells were fused with Sp2/0 myeloma cells. The antibody binds to the CC chemokine receptor CCR9 (GPR-9-6) and blocks the binding of the thymus-expressed chemokine (TECK) ligand to the receptor.

Propagation: ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose and supplemented with 50 ng/ml mouse recombinant Interleukin-6 (IL-6) and 10% fetal bovine serum

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Temperature: 37.0°C

Subculturing: LS129-3C3-E3-1 [3C3]細胞Protocol: Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 3 X 10(4) viable cells/ml.

Interval: Maintain cultures at a cell concentration between 3 X 10(4) and 1 X 10(6) cells/ml.

Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)

Preservation: Freeze medium: Complete growth medium supplemented with 7.5% (v/v) DMSO

Storage temperature: liquid nitrogen vapor phase

Doubling Time: 18 hours

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002

recommended serum:ATCC 30-2020

References: 71176: Zabel BA, et al. LS129-3C3-E3-1 [3C3]細胞Human G protein-coupled receptor GPR-9-6/CC chemokine receptor 9 is selectively expressed on intestinal homing T lymphocytes, mucosal lymphocytes, and thymocytes and is required for thymus-expressed chemokine-mediated chemotaxis.. J. Exp. Med. 190: 1241-1256, 1999. PubMed: 10544196

71178: Andrew DP, et al. Anti-GPR-9-6 antibodies and methods of identifying agents which modulate GPR-9-6 function. US Patent 6,329,159 dated Dec 11 2001