Genox 3.53細(xì)胞
運輸方式： 凍存運輸
是否是腫瘤細(xì)胞： 0
物種來源： 小鼠
ATCC Number： HB-103?
細(xì)胞形態(tài)： **樣
**類型： IgG1
生長狀態(tài)： 懸浮生長
數(shù)量： 大量
Genox 3.53細(xì)胞Designations: Genox 3.53
Depositors: P Parham
Isotype: IgG1
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products:Genox 3.53細(xì)胞 immunoglobulin; monoclonal antibody; against DQw1 (DC1, HLA DR)
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Comments: Animals were immunized with glycoproteins from the Bristol 8 human B lymphoblastoid cell line.
Spleen cells were fused with NS-1 myeloma cells.
The antibody reacts with the DQw1 (DC10) determinant of the HLA D region.
Genox 3.53細(xì)胞Tested and found negative for ectromelia virus (mousepox).
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
References: 951: Brodsky FM, et al. Genox 3.53細(xì)胞Monoclonal antibodies to HLA -- DRw determinants. Tissue Antigens 16: 30-48, 1980. PubMed: 6162228
