TR 310細胞
生長狀態： 懸浮生長
ATCC Number： HB-219?
運輸方式： 凍存運輸
數量： 大量
是否是腫瘤細胞： 0
物種來源： 大鼠
細胞形態： **樣
**類型： IgG2b
Designations: TR 310
TR 310細胞Depositors: IL Weissman
Isotype: IgG2b
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Rattus norvegicus (B cell); Mus musculus (myeloma) deposited as rat (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products: TR 310細胞immunoglobulin; monoclonal antibody; against mouse T cell receptor
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments: Animals were immunized with the Tk1 murine T cell lymphoma.
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
TR 310細胞The antibody reacts with a deteminant on the mouse T cell antigen receptor.
It recognizes a determinant encoded by the Vb7 (V beta 7) gene segment.
Propagation: ATCC complete growth medium: RPMI 1640 medium with 2 mM L-glutamine, 1 mM sodium pyruvate and 0.05 mM 2-mercaptoethanol, 90%; fetal bovine serum, 10%
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. TR 310細胞Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
