2E6細胞
生長狀態： 懸浮生長
運輸方式： 凍存運輸
細胞形態： **樣
**類型： IgG
ATCC Number： HB-226?
數量： 大量
是否是腫瘤細胞： 0
物種來源： 倉鼠
Designations: 2E6
Depositors: RM Steinman
2E6細胞Isotype: IgG
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Cricetulus migratorius (B cell); Mus musculus (myeloma) deposited as hamster, Armenian (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: Cell Type: hybridoma:lymphoblast B lymphocyte;
Cellular Products: immunoglobulin; monoclonal antibody; against mouse beta 2 leukocyte integrins (CD18)
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Comments: 2E6細胞Animals were immunized with mouse dendritic cells.
Spleen cells were fused with Sp2/0 myeloma cells.
The antibody reacts precipitates a 150000/90000 dalton CD11/CD18 heterodimer (see also ATCC HB-225) from murine leukocytes.
2E6 blocks the binding function of LFA-1 (CD11a) and CR3/Mac-1 (CD11b).
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium:
fetal bovine serum to a final concentration of 10%
0.05 mM 2-mercaptoethanol
Temperature: 37.0°C
Subculturing: Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 X 10 exp5 viable cells/ml.Maintain cell density between 1 X 10 exp5 and 1 X 10 exp6 viable cells/ml.
2E6細胞Medium Renewal: Every 2 to 3 days
Preservation: Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor temperature
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001
recommended serum:ATCC 30-2020
References: 1793: Metlay JP, et al. The distinct leukocyte integrins of mouse spleen dendritic cells as identified with new hamster monoclonal antibodies. J. Exp. Med. 171: 1753-1771, 1990. 2E6細胞PubMed: 2185332
