Gr431細(xì)胞

數(shù)量： 大量

運(yùn)輸方式： 凍存運(yùn)輸

細(xì)胞形態(tài)： **樣

**類型： IgM

ATCC Number： HB-8575?

生長狀態(tài)： 懸浮生長

是否是腫瘤細(xì)胞： 0

物種來源： 人

Designations: Gr431

Gr431細(xì)胞Depositors: Sloan-Kettering Institute for Cancer Research

Isotype: IgM

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: human (B cell); mouse (myeloma)

Morphology: lymphoblast

Source: Cell Type: hybridoma: B lymphocyte;

Cellular Products: Gr431細(xì)胞immunoglobulin; monoclonal antibody; against an intracellular antigen expressed in human malignant cells (cancer, tumor)

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Comments: Lymphocytes were obtained from the regional lymph nodes of a patient who had breast cancer.

Lymphocytes were fused with NS-1 myeloma cells.

Gr431細(xì)胞Propagation: ATCC complete growth medium: RPMI 1640 medium with 0.1 mM hypoxanthine and 0.016 mM thymidine, 90%; fetal bovine serum, 10%

Subculturing: Medium Renewal: Every 2 to 3 days

Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.

Preservation: Culture medium, 90%; DMSO, 10%

References: 3897: Thomson TM, et al.Gr431細(xì)胞 Human monoclonal antibodies to cell surface antigens. US Patent 4,695,538 dated Sep 22 1987