Y3-Ag 1.2.3細(xì)胞
品系： Lou
ATCC Number： CRL-1631?
相關(guān)**： 多發(fā)性骨髓瘤
數(shù)量： 大量
細(xì)胞類型： B**細(xì)胞
是否是腫瘤細(xì)胞： 0
物種來源： 大鼠
生長(zhǎng)狀態(tài)： 懸浮生長(zhǎng)
運(yùn)輸方式： 凍存運(yùn)輸
細(xì)胞形態(tài)： **樣
**類型： kappa light chain
Y3-Ag 1.2.3細(xì)胞Designations: Y3-Ag 1.2.3
Depositors: K Yamada
Isotype: kappa light chain
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Rattus norvegicus deposited as Rattus sp.
Morphology: lymphoblast
Source: Disease: plasmacytoma; myeloma
Strain: Lou
Cell Type: B lymphoblast;
Cellular Products: immunoglobulin
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Y3-Ag 1.2.3細(xì)胞Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Restrictions: the depositor has provided these cells for research purposes only and with the following restrictions: (1) no distribution should be made to third parties, (2) any proposed commercial use must be negotiated with the National Research Development Corporation, 101 Newington Causeway, London SE1 6BU, (3) all papers reporting any use of these or derived clones should make direct reference to the original publication (Nature 277:131-133, 1979)
Comments: This is a derivative of the S210 myeloma developed by C. Milstein.
No commercial use or third party distribution is permitted without the concurrence of C. Milstein.
The cells are resistant to 8-azaguanine, are HAT sensitive and can be used as a fusion partner for rat B cells to make rat - rat hybridomas.
Tested and found negative for ectromelia virus (mousepox).
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Y3-Ag 1.2.3細(xì)胞Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 X 10 exp5 viable cells/ml.
Maintain cell density between 1 X 10 exp5 and 1 X 10 exp6 viable cells/ml.
Preservation: culture medium 95%; DMSO, 5%
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2020
References: 1090: Galfre G, et al. Rat x rat hybrid myelomas and a monoclonal anti-Fd portion of mouse IgG. Nature 277: 131-133, 1979. PubMed: 310519
26115: Bazin H, et al. Transplantable immunoglobulin-secreting tumours in rats. I. General features of LOU-Ws1 strain rat immunocytomas and their monoclonal proteins. Int. J. Cancer 10: 568-580, 1972.Y3-Ag 1.2.3細(xì)胞 PubMed: 4668496
