YPEN-1細(xì)胞
年限： 8 weeks
物種來源： 大鼠
是否是腫瘤細(xì)胞： 0
細(xì)胞形態(tài)： 上皮樣
數(shù)量： 大量
細(xì)胞類型： 其他細(xì)胞類型
組織來源： endothelium
品系： Copenhagen
器官來源： 前列腺
運輸方式： 凍存運輸
生長狀態(tài)： 貼壁生長
相關(guān)**： 正常
YPEN-1細(xì)胞ATCC Number： CRL-2222?
Designations: YPEN-1
Depositors: K Yamakazi, K Pienta
Biosafety Level: 2 [Cells contain polyomavirus DNA sequences ]
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Rattus norvegicus deposited as Rattus sp.
Morphology: epithelial
Source: Organ: prostate
Strain: Copenhagen
Tissue: endothelium
Disease: normal
Cell Type:YPEN-1細(xì)胞 immortalized with adenovirus 12 - SV40 virus hybrid (Ad12-SV40)
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Isolation: Isolation date: 1993
Tumorigenic: No
Age: 8 weeks
Gender: male
Comments: The YPEN-1 cell line was originated in 1993 from prostate cells of 8 week old Copenhagen male rats.
Cells were cultured in Endothelium Isolation Medium and immortalized by infection with Adenovirus12 SV40 hybrid virus.
The cells stain positively for but are non-producers of SV40 T-antigen.
YPEN-1 cells demonstrate acetylated low density lipoprotein (Dil-Ac-LDL) uptake as an endothelial marker.
They exhibit positive staining for endothelin and for a monoclonal antibody to rat endothelium (MRC OX-43).
They express Integrin a6 1 and Integrin 3 on their plasma membrane, and demonstrate tube formation in Matrigel.
Propagation: YPEN-1細(xì)胞ATCC complete growth medium: Minimum essential medium (Eagle) with 2 mM L-glutamine and Earle's BSS adjusted to contain 1.5 g/L sodium bicarbonate, 0.1 mM non-essential amino acids, and 1.0 mM sodium pyruvate, supplemented with 0.03 mg/ml heparin, 95%; fetal bovine serum, 5%
Temperature: 37.0°C
Subculturing: Protocol:
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37?C to facilitate dispersal.
Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37?C.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended
Medium Renewal: Every 2 to 3 days
Preservation: YPEN-1細(xì)胞Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Doubling Time: 26 hrs
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003
recommended serum:ATCC 30-2020
References: 49801: Yamazaki K, et al. Establishment of immortalized Copenhagen rat prostate endothelial cell lines. In Vivo 9: 421-426, 1995. PubMed: 8900918
