2D7F10細胞
是否是腫瘤細胞： 0
物種來源： 小鼠
生長狀態： 懸浮生長
數量： 大量
運輸方式： 凍存運輸
細胞形態： **樣
**類型： IgA
2D7F10細胞ATCC Number： CRL-2025?
Designations: 2D7F10
Depositors: KW Ruby
Isotype: IgA
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology: 2D7F10細胞lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products: immunoglobulin; monoclonal antibody; against Leptospira pomona type kennewicki
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Tumorigenic: Yes
Comments: Animals were immunized with.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
2D7F10細胞The antibody binds to agglutinates L. interogans serovar pomona type kennewicki, and binds to type kennewicki antigen in ELISA.
It can be used to identify type kennewicki in veterinary biologicals.
Propagation: ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 92.5%; horse serum, 5%; fetal bovine serum, 2.5%
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
References: 24407: . . 2D7F10細胞Proceedings of the 94th Annual Meeting of the United States Animal Health Association, Denver, CO, : 290-294, 1990.
