204-4細胞

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產品名稱: 204-4細胞

產品型號: 204-4

產品展商: HZbscience

產品文檔: 無相關文檔

簡單介紹

204-4細胞應如何避免細胞污染，細胞污染的種類可分成**、酵母菌、霉菌、病毒和霉漿菌。主要的污染原因為無菌操作技術不當、操作室環境不佳、污染之血清和污染之細胞等。嚴格之無菌操作技術、清潔的環境、與品質良好之細胞來源和培養基配制是減低污染之*好方法。204-4細胞何時須更換培養基？視細胞生長密度而定，或遵照細胞株基本數據上之更換時間，按時更換培養基即可。

204-4細胞 的詳細介紹

204-4細胞

數量：大量

生長狀態：懸浮生長

運輸方式：凍存運輸

ATCC Number： HB-185?

細胞形態： **樣

**類型： IgG2a; kappa light chain

是否是腫瘤細胞： 0

物種來源：小鼠

204-4細胞Designations: 204-4

Depositors: BS Prabhakar

Isotype: IgG2a; kappa light chain

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)

Morphology: lymphoblast

Source: Cell Type: hybridoma: B lymphocyte;

Cellular Products: 204-4細胞immunoglobulin; monoclonal antibody; against coxsackievirus B4 (CB4); neutralizing

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Tumorigenic: Yes

Comments: Animals were immunized with coxsackievirus B4 (JVB strain, ATCC VR 184).

Spleen cells were fused with P3X63Ag8.653 myeloma cells.

The antibody reacts with a highly conserved epitope on the JVB strain of CB4.

Neutralizes most human isolates of CB4 tested.

204-4細胞Tested and found negative for ectromelia virus (mousepox).

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Subculturing: Medium Renewal: Every 2 to 3 days

Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.