Ra Bot細胞
ATCC Number： CRL-1265?
運輸方式： 凍存運輸
相關**： 馬凡氏綜合征
細胞形態： 成纖維樣
是否是腫瘤細胞： 0
物種來源： 人
數量： 大量
生長狀態： 貼壁生長
Ra Bot細胞年限： 8 years
器官來源： 皮膚
Designations: Ra Bot
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens
Morphology: fibroblast
Source: Organ: skin
Disease: Ra Bot細胞Marfan syndrome
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Age: 8 years
Gender: female
Comments: The tissue donor suffered from a severe form of Marfan syndrome.
There was no prior family history of Marfan syndrome.
Propagation: Ra Bot細胞ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended
Medium Renewal: 2 to 3 times per week
Remove spent medium, add fresh 0.25% trypsin for 3 to 5 minutes, remove trypsin and let the culture sit at 37C for 10 to 20 minutes. Add fresh medium, aspirate and dispense into new flasks. Ra Bot細胞Subculture every 7 to 9 days.
