UCD/PR 10.11細胞
生長狀態： 懸浮生長
ATCC Number： HB-8694?
是否是腫瘤細胞： 0
物種來源： 小鼠
運輸方式： 凍存運輸
數量： 大量
細胞形態： **樣
**類型： IgG
Designations: UCD/PR 10.11
UCD/PR 10.11細胞Depositors: Regents of the University of California
Isotype: IgG
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products:UCD/PR 10.11細胞 immunoglobulin; monoclonal antibody; against type 8 and type 18 cytokeratins
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments: Animals were immunized with soluble extracellular cytokeratin.
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
The antibody reacts type 8 and type 18 cytokeratins in immunoblots.
The antibody can be used to detect cytokeratin in formalin or B-5 fixed paraffin sections.
The antibody primarily reacts with protein antigens in ductal portions of glands and with primary and metastatic adenocarcinomas.
UCD/PR 10.11細胞Radioactively labeled UCD/PR 10.11 antibody can also be used to detect soluble extracellular cytokeratin in a quantitative sandwich radioimmunoassay.
Propagation: ATCC complete growth medium: Temperature: 37.0°C
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Preservation: culture medium 95%; DMSO, 5%
Related Products: recommended serum:ATCC 30-2020
Recommended medium UCD/PR 10.11細胞(without the additional supplements or serum described under ATCC Medium):ATCC 46-X
References: 3931: Cardiff RD, et al. Cytokeratin tumor markers and assays for their detection. US Patent 4,775,620 dated Oct 4 1988
