S49.1G.3 PHA.100/0細(xì)胞
細(xì)胞類型： T**細(xì)胞
是否是腫瘤細(xì)胞： 0
物種來(lái)源： 小鼠
品系： BALB/c
運(yùn)輸方式： 凍存運(yùn)輸
數(shù)量： 大量
細(xì)胞形態(tài)： **樣
ATCC Number： TIB-35?
相關(guān)**： **瘤
S49.1G.3 PHA.100/0細(xì)胞生長(zhǎng)狀態(tài)： 懸浮生長(zhǎng)
Designations: S49.1G.3 PHA.100/0 (replaces S49.1G3 PHA.12P)
Depositors: P Ralph
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus
Morphology: lymphoblast
Source: Disease: lymphoma
Strain: BALB/c
Cell Type: S49.1G.3 PHA.100/0細(xì)胞T lymphocyte;
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Antigen Expression: H-2d; Thy-1.2; TL
Comments: This variant of S49.1G.3 (ATCC TIB-34) was selected for resistance to 5 mcg/ml phytohemagglutinin.
The cells are also resistant to 6-thioguanine and sensitive to thymidine, and hydrocortisone.
Tested and found negative for ectromelia virus (mousepox).
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. S49.1G.3 PHA.100/0細(xì)胞To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
References: 1130: Ralph P, et al. Independence of theta and TL surface antigens and killing by thymidine, cortisol, phytohemagglutinin, and cyclic AMP in a murine lymphoma. Biochem. Biophys. Res. Commun. 55: 1085-1091, 1973. PubMed: 4358928
22154: Horibata K, Harris AW. Mouse myelomas and lymphomas in culture. S49.1G.3 PHA.100/0細(xì)胞Exp. Cell Res. 60: 61-77, 1970. PubMed: 5439579
