S49.1TB.2細(xì)胞
生長(zhǎng)狀態(tài)： 懸浮生長(zhǎng)
細(xì)胞類型： T**細(xì)胞
是否是腫瘤細(xì)胞： 0
物種來(lái)源： 小鼠
ATCC Number： TIB-30?
相關(guān)**： **瘤
運(yùn)輸方式： 凍存運(yùn)輸
品系： BALB/c
細(xì)胞形態(tài)： **樣
S49.1TB.2細(xì)胞數(shù)量： 大量
Designations: S49.1TB.2
Depositors: A Harris
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus
Morphology: lymphoblast
Source: Disease: lymphoma
Strain: BALB/c
Cell Type: S49.1TB.2細(xì)胞T lymphocyte;
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Antigen Expression: H-2d; Thy-1.2; TL
Comments: This line was derived from S49.1 (ATCC TIB-28) by selecting for resistance to 10 mM thymidine and 0.1 mM 5-bromo-2'-deoxyuridine.
Like the parent line, the cells are sensitive to 0.001 mM cortisol (hydrocortisone), 5 mcg/ml phytohemagglutinin and 0.01 mM 6-thioguanine.
Tested and found negative for ectromelia virus (mousepox).
Propagation: ATCC complete growth medium: Dulbecco's modified Eagle's medium, 90%; horse serum, 10%
Subculturing: S49.1TB.2細(xì)胞Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
References: 1130: Ralph P, et al. Independence of theta and TL surface antigens and killing by thymidine, cortisol, phytohemagglutinin, and cyclic AMP in a murine lymphoma. Biochem. Biophys. Res. Commun. 55: 1085-1091, 1973. PubMed: 4358928
22154: Horibata K, Harris AW. Mouse myelomas and lymphomas in culture. Exp. Cell Res. 60: 61-77, 1970. PubMed: 5439579
22241: Schiller JG, et al. S49.1TB.2細(xì)胞Glucocorticoid receptors in lymphoma cells in culture: relationship to glucocorticoid killing activity. Science 171: 189-191, 1971. PubMed: 4395230
