201-45E9細(xì)胞
是否是腫瘤細(xì)胞： 0
物種來源： 小鼠
細(xì)胞形態(tài)： **樣
**類型： IgG1 and IgG2b
ATCC Number： CRL-2670?
運(yùn)輸方式： 凍存運(yùn)輸
生長(zhǎng)狀態(tài)： 懸浮生長(zhǎng)
數(shù)量： 大量
Designations: 201-45E9
Depositors: National Cancer Institute
201-45E9細(xì)胞Isotype: IgG1 and IgG2b
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products: immunoglobulin; monoclonal antibody; against a v-src synthetic oncogene peptide
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments: 201-45E9細(xì)胞Animals were immunized with a synthetic polypeptide with the sequence LMCQCWRKDPEERPTF corresponding to peptides 494 to 509 of the v-src oncogene (formerly 273 to 284).
Spleen cells were fused with Sp2/0 myeloma cells.
In ELISA testing, the antibody cross reacts with the homologous region of FES, v-mos, erb B, v-src, FGR, yes, mil, FMS, abl, ros, lck, and MET. Weak activity was detected for PKS and fps.
Binding was not detected for c-mos, MOS, SRC, ros, rel, FMS, HIR, TRK, sea, and kit. In immunoblot testing, a protein of approximately 40 kd is detected in fetal brain.
The IgG1 reactivity of the antibody with the v-src peptide (494 to 509) was confirmed by ELISA.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: 201-45E9細(xì)胞Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 X 10 exp5 viable cells/ml.
Maintain cell density between 1 X 10 exp5 and 1 X 10 exp6 viable cells/ml.
Preservation: culture medium 95%; DMSO, 5%
Related Products: Recommended medium 201-45E9細(xì)胞(without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2020
