S6F1細胞
生長狀態： 懸浮生長
細胞形態： **樣
**類型： IgG1
是否是腫瘤細胞： 0
物種來源： 小鼠
ATCC Number： HB-9579?
運輸方式： 凍存運輸
數量： 大量
S6F1細胞Designations: S6F1
Depositors: Dana Farber Cancer Inst.
Isotype: IgG1
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology:S6F1細胞 lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products: immunoglobulin; monoclonal antibody; against a subset of human CD8 (LFA-1) positive T cells
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Comments: Animals were immunized with the 1670 cell line produced by immortalizing spleen cells from a whitelip tamarin with Herpesvirus saimiri.
Spleen cells were fused with P3/NSI/1-Ag4-1 myeloma cells.
S6F1細胞The antibody recognizes a subset of CD8 positive T cells that have cytotoxic effector activity.
The S6F1 epitope is absent on CD8 positive suppressor cells.
Propagation: ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 2 mM L-glutamine, 0.1 mM hypoxanthine, and 0.032 mM thymidine, 80%; heat-inactivated fetal bovine serum, 10%; heat-inactivated horse serum, 10%
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 2 X 10 exp5 and 1 X 10 exp6 cells/ml.
References: 21964: S6F1細胞Schlossman SF, Morimoto C. Monoclonal antibody specific to a novel epitope of the LFA-1 antigen of human T lymphocytes. US Patent 5,002,869 dated Mar 26 1991
