UV5 (UV sensitive mutant of CHO)細胞

是否是腫瘤細胞： 0

物種來源： 倉鼠

細胞形態： 上皮樣

ATCC Number： CRL-1865?

運輸方式： 凍存運輸

生長狀態： 單層、懸浮

數量： 大量

器官來源： 卵巢

Designations: UV5 (UV sensitive mutant of CHO)

UV5 (UV sensitive mutant of CHO)細胞Depositors: LH Thompson

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: monolayer and suspension

Organism: Cricetulus griseus

Morphology: epithelial-like

Source: Organ: ovary

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Gender: female

Comments: UV5 (UV sensitive mutant of CHO)細胞This line is a derivative of the CHO-K1 cell line (see ATCC CCL-61).

UV5 is a UV sensitive line derived from AA8 (see ATCC CRL-1859).

The line is defective in nucleotide excision repair, is sensitive to bulky adduct mutagens and belongs to excision repair complementation group 2.

Propagation: ATCC complete growth medium: Alpha minimum essential medium without ribonucleosides and deoxyribonucleosides, 90%; fetal bovine serum, 10%

Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:12 is recommended

Medium Renewal: 2 to 3 times per week

To subculture attached cells, remove the medium and rinse the cells with fresh 0.25% trypsin, 0.03% EDTA.

Let the culture sit at 37C until the cells detach (about 5 minutes).

Add fresh medium, aspirate and transfer to new flasks.

The suspended cells are viable and can be used to start new cultures.

Doubling Time: 13 hrs

References: 1767: Thompson LH, et al. Repair of DNA adducts in asynchronous CHO cells and the role of repair in cell killing and mutation induction in synchronous cells treated with 7-bromomethylbenz[a]anthracene. Somatic Cell Mol. Genet. 10: 183-194, 1984. PubMed: 6584989

1768: Thompson LH, et al. UV5 (UV sensitive mutant of CHO)細胞Genetic diversity of UV-sensitive DNA repair mutants of Chinese hamster ovary cells. Proc. Natl. Acad. Sci. USA 78: 3734-3737, 1981. PubMed: 6943579

1769: Hoy CA, et al. Defective DNA cross-link removal in Chinese hamster cell mutants hypersensitive to bifunctional alkylating agents. Cancer Res. 45: 1737-1743, 1985. PubMed: 3919945

1770: Busch D, et al. Summary of complementation groups of UV-sensitive CHO cell mutants isolated by large-scale screening. Mutagenesis 4: 349-354, 1989. PubMed: 2687628

32479: Bessho T, et al. Initiation of DNA interstrand cross-link repair in humans: the nucleotide excision repair system makes dual incisions 5" to the cross-linked base and removes a 22- to 28-nucleotide-long damage-free strand. Mol. Cell. Biol. 17: 6822-6830, 1997. PubMed: 9372913

32924: Reardon JT, et al. Isolation and characterization of two human transcription factor IIH (TFIIH)-related complexes: ERCC2/CAKand TFIIH. Proc. Natl. Acad. Sci. USA 93: 6482-6487, 1996. PubMed: 8692841

58395: Thompson LH, et al. Hypersensitivity to mutation and sister-chromatid-exchange induction in CHO cell mutants defective in incising DNA containing UV lesions. Somatic Cell Genet. 8: 759-773, 1982. PubMed: 7163954

58396: Thompson LH, et al. UV5 (UV sensitive mutant of CHO)細胞A screening method for isolating DNA repair-deficient mutants of CHO cells. Somatic Cell Genet. 6: 391-405, 1980. PubMed: 7404270